基于宏基因组学研究分析农药对宁夏枸杞地微生物的影响  

Analysis of the Effects of Pesticides on Microbial Diversity of Lycium chinense L.in Ningxia Based on Macrogenomics

作　　者：林文星 马鹏生[1] 王芳[2] 周丽[1] 刘成[1] 吴秀丽[1] LIN Wenxing;MA Pengsheng;WANG Fang;ZHOU Li;LIU Cheng;WU Xiuli(College of Pharmacy,Ningxia Medical University/Key Laboratory of Ningxia Ethnomedicine Modernization,Ministry of Education at Ningxia Medical University,Yinchuan,Ningxia 750004;Institute of Plant Protection,Ningxia Academy of Agriculture and Forestry Sciences,Yinchuan,Ningxia 750004)

机构地区：[1]宁夏医科大学药学院,宁夏少数民族医药现代化教育部重点实验室,宁夏银川750004 [2]宁夏农林科学院植物保护研究所,宁夏银川750004

出　　处：《北方园艺》2023年第1期98-105,共8页Northern Horticulture

基　　金：宁夏回族自治区重点研发计划资助项目(2020BFG02006);宁夏回族自治区自然科学基金资助项目(2022AAC03198)。

摘　　要：以宁夏3个不同产区枸杞地喷施农药前后土壤样本为研究对象,采用Illumina测序平台对其进行宏基因组高通量测序,研究农药对宁夏枸杞地土壤微生物群落的影响,以期为枸杞地生态环境保护、农药降解菌种的开发利用提供参考依据。结果表明:共鉴定出89个门类、2 102个属及8 337个种。不同样地香农指数在施用农药后均有所降低,农药使枸杞地土壤的微生物群落多样性下降。农药的使用增加了枸杞地土壤主要菌门中变形菌门、Candidatus Tectomicrobia的相对丰度,以及主要菌属Gaiella,Candidatus entotheonella,亚硝化螺菌属(Nitrosospira),Euzebya,Nitriliruptor的相对丰度。除此之外,农药的使用还增加了某些致病菌属的相对丰度。功能预测枸杞地土壤微生物主要涉及全局和总览图、碳水化合物代谢、氨基酸代谢等45个子功能,表现出功能上的丰富性,农药的使用降低了土壤微生物的整体代谢能力。Taking the soil samples before and after pesticide spraying in the Lycium fields of three different production areas in Ningxia as the research object, the Illumina sequencing platform to carry out the high throughput sequencing of their macrogenomes to study the impact of pesticides on the soil microbial community in the Lycium fields in Ningxia, in order to provide reference for the ecological environment protection of the Lycium fields and the development and utilization of pesticide degrading bacteria.The results showed that there were 89 phyla, 2 102 genera and 8 337 species identified.Pesticides led to the reduction of Shannon index in different areas as well as the soil microbial community diversity of Lycium chinense L..The pesticides increased the relative abundance of the major phyla of the soil in the phylum Aspergillus, Candidatus Tectomicrobia, as well as the major genera Gaiella, Candidatus entotheonella, Nitrosospira, Euzebya and Nitriliruptor.Besides, it also increased the relative abundance of some pathogenic bacteria genera.Functional predictions of soil microorganisms were mainly involved in 45 sub-functions such as global and loverview maps, carbohydrate metabolismand amino acid metabolism, showing functional richness.In short, the use of pesticide reduced the overall metabolic capacity of soil microorganisms.

关 键 词：枸杞 农药 微生物 宏基因组测序 

分 类 号：S567[农业科学—中草药栽培]