大豆荚膜过氧化物酶催化硫醚反应动力学模型  

Kinetic Model of Thioether Reaction Catalyzed by Soybean Pod Peroxidase

作　　者：刘翠 邓雅姗 许慧 薛晓 李楷原 王繁业[1] LIU Cui;DENG Yashan;XU Hui;XUE Xiao;LI Kaiyuan;WANG Fanye(College of Chemical Engineering,Qingdao University of Science and Technology,Qingdao 266042,China)

机构地区：[1]青岛科技大学化工学院,山东青岛266042

出　　处：《湖南科技大学学报（自然科学版）》2022年第4期110-118,共9页Journal of Hunan University of Science And Technology：Natural Science Edition

基　　金：国家自然科学基金资助项目(21546004;21576145)。

摘　　要：用大豆荚膜过氧化物酶(Soybean Pod Peroxidase,SPP)在微乳液中催化不对称氧化反应,在酶活性为3200 U/mL,50℃时反应5 h,产率为91.56%,对映体过量值为96.08%.SPP催化奥美拉唑硫醚不对称氧化反应生成S-奥美拉唑,其反应机制包括酶催化的SPP-I双电子还原反应、SPP-I单电子转移反应和非酶催化的化学反应.建立的动力学模型很好地拟合了试验数据,其平均相对偏差为5.44%.酶促反应动力学机制为带底物产物抑制的乒乓机制,而化学反应遵循幂律,过氧化氢产生底物抑制,S-奥美拉唑产生产物抑制.In water-in-oil microemulsions the asymmetric oxidation catalyzed by soybean pod peroxidase(SPP)to form esomeprazole is carried out with the yield of 91.56%and e.e.of 96.08%at the activity of SPP of 3200 U/mL,and 50℃for 5 h.The mechanism with a two-electron reduction of SPP-I is accompanied with a single-electron transfer to SPP-I and nonenzymatic reactions.With 5.44%of the average relative deviation,a kinetic model fitting experimental data very well is developed.The enzymatic reactions may follow ping-pong mechanism with substrate inhibition of hydrogen peroxide and produce the inhibition of esomeprazole,while nonenzymatic reactions may follow a power law.

关 键 词：动力学模型 大豆荚膜过氧化物酶 不对称亚砜氧化 手性亚砜 底物抑制 

分 类 号：Q814.9[生物学—生物工程]