基于气体交换及OJIP光化学参数评估甜菜光温适应性及鉴定指标筛选  

Assessment of light-temperature adaptation of sugar beet based on gas exchange and OJIP photochemical parameters and screening of its identification indicators

作　　者：李思忠 张立明[1] 林明[1] 高卫时[1] 白晓山[1] 刘军[1] 董心久[1] 沙红[1] 曹禹 LI Si-zhong;ZHANG Li-ming;LIN Ming;GAO Wei-shi;BAI Xiao-shan;LIU Jun;DONG Xin-jiu;SHA Hong;CAO Yu(Research Institute of Economic Crops,Xinjiang Academy of Agricultural Sciences,Urumqi 830091,China;Yili Prefecture Institute of Agricultural Sciences,Yining 839300,Xinjiang,China)

机构地区：[1]新疆农业科学院经济作物研究所,乌鲁木齐830091 [2]伊犁州农业科学研究所,新疆伊宁839300

出　　处：《生态学杂志》2023年第1期248-256,共9页Chinese Journal of Ecology

基　　金：新疆维吾尔自治区科技成果转化示范专项(2020B007);国家糖料产业技术体系岗位(CARS-170108);新疆维吾尔自治区重点研发项目(2022B02002-2)资助。

摘　　要：高温和强光是夏季最常见的非生物胁迫,严重影响作物产量及果实品质。探明不同甜菜品种在高温强光复合环境条件下的光合性能及抗逆指标筛选,为甜菜耐逆品种培育及高产栽培提供理论依据。以8个甜菜品种为材料,在甜菜块根膨大期,评估了不同甜菜品种在强光高温复合胁迫下叶片的光合性能,测定了甜菜叶片的气体交换和叶绿素荧光动力学参数等26个指标,综合评价了甜菜在复合环境约束下的耐逆性。结果表明:不同甜菜品种对高温强光复合胁迫的反应不同;通过主成分分析将26个单项指标转化为4个相互独立的综合指标,涵盖了全部数据95%的信息量;利用隶属函数法和聚类分析将8个甜菜品种划分为3类;采用逐步回归分析方法建立甜菜耐高温强光复合胁迫的评价数学模型:D=0.745φ_(Eo)-0.320,φ_(Eo)可作为鉴定甜菜品种耐高温强光复合胁迫强弱的辅助指标。强耐高温强光复合胁迫的甜菜品种具有更高的光系统Ⅱ(PSⅡ)单元之间能量连接性、PSⅡ和光系统Ⅰ(PSⅠ)之间的光合电子传递能力,对放氧复合体活性的损害更小,PSⅡ的稳定性更好。High temperature and intense light are the most common abiotic stresses in summer, seriously threatening crop yield and fruit quality. To provide a theoretical basis for stress tolerant variety breeding and high yield cultivation of sugar beet, we explored the photosynthetic performance of different varieties under compound environmental constraints of high temperature and intense light, to screen out resistance indicators. The leaf photosynthetic performance of eight sugar beet varieties under the compound stress of strong light and high temperature was evaluated at the tuber expansion stage. A total of 26 indicators, including gas exchange and chlorophyll fluorescence kinetic parameters of sugar beet leaves, were measured. The stress tolerance of sugar beet under combined environmental constraints was comprehensively evaluated. The results showed that different sugar beet varieties responded differently to combined stresses of high temperature and intense light. The 26 indicators were transformed into four independent composite indicators by principal component analysis, covering 95% of the total data information. The eight sugar beet varieties were classified into three categories by using the membership function and cluster analysis. An optimal evaluation mathematical model for sugar beet tolerance to combined stress of high temperature and intense light was established by using stepwise regression analysis: D=0.745φ_(Eo)-0.320(D, the value of comprehensive evaluation of stress resistance;φ_(Eo), the quantum yield of absorbed energy used for electron transfer). φ_(Eo)can be used as an auxiliary index to identify the strength of sugar beet varieties to tolerate combined stress of high temperature and intense light. Sugar beet varieties with strong tolerance to combined stress of high temperature and intense light have higher energy connectivity between photosystem Ⅱ(PSⅡ) units, higher photosynthetic electron transfer capacity between PSⅡ and photosystem Ⅰ(PSⅠ), less damage to the activity of the o

关 键 词：甜菜 气体交换 光化学参数 鉴定指标 多元分析 

分 类 号：S566.3[农业科学—作物学]