干扰素治疗前后慢性乙型肝炎患者外周血淋巴细胞和中性粒细胞表面CD64表达变化观察  

Changes of CD64 expression on surface of peripheral blood lymphocytes and neutrophils in patients with chronic hepatitis B before and after interferon treatment

作　　者：尚鹏程 许娇 钟晶 毕雷 SHANG Pengcheng;XU Jiao;ZHONG Jing;BI Lei(Clinical Laboratory,Public Health Clinical Center of Chengdu,Chengdu 610000,China)

机构地区：[1]成都市公共卫生临床医疗中心检验科,成都610000

出　　处：《山东医药》2023年第3期22-25,共4页Shandong Medical Journal

基　　金：四川省卫生健康委员会基金项目(16PJ083)。

摘　　要：目的通过观察干扰素治疗前后慢乙肝(CHB)患者外周血淋巴细胞和中性粒细胞表面CD64表达量的变化,明确外周血淋巴细胞和中性粒细胞表面CD64表达量与HBsAg、HBV-DNA、ALT水平的相关性,为下一步探讨外周血淋巴细胞和中性粒细胞表面CD64是否可用于评估干扰素治疗效果提供参考资料。方法收集57例CHB患者,其中e抗原阳性组33例、e抗原阴性组24例,均采用干扰素治疗48周。另选同期健康体检者16例为对照组。采用流式细胞术检测外周血淋巴细胞及中性粒细胞表面CD64,干扰素治疗前,治疗8、12、24、48周时比较各组患者外周血淋巴细胞及中性粒细胞表面CD64表达量。采用Pearson相关分析干扰素治疗不同时间CHB患者外周血淋巴细胞及中性粒细胞表面CD64表达量与HBsAg、HBV-DNA、ALT水平的相关性。结果干扰素治疗前,CHB患者外周血淋巴细胞表面CD64和中性粒细胞表面CD64表达量均高于对照组(P均<0.05)。与对照组比较,e抗原阳性组及e抗原阴性组淋巴细胞表面CD64表达量高(P<0.05),e抗原阳性组与e抗原阴性组比较,P>0.05;与对照组比较,e抗原阳性组中性粒细胞表面CD64表达量高(P<0.05),e抗原阳性组高于e抗原阴性组(P<0.05)。干扰素治疗后,CHB患者淋巴细胞表面CD64和中性粒细胞表面CD64表达量均下降,与干扰素治疗前比较,从干扰素治疗24周开始,淋巴细胞表面CD64表达量差异有统计学意义(P<0.05),从干扰素治疗48周开始中性粒细胞表面CD64表达量差异有统计学意义(P<0.05)。干扰素治疗不同时间,CHB患者淋巴细胞表面CD64和中性粒细胞表面CD64表达量与HBsAg、ALT水平均有相关性(P均<0.05),与HBV-DNA水平无相关性(P>0.05)。结论与健康对照比,CHB患者外周血淋巴细胞表面和中性粒细胞表面CD64表达量升高,干扰素治疗后淋巴细胞表面CD64和中性粒细胞表面CD64表达量下降;淋巴细胞表面和中性粒细胞表面CD64表达量与HBsObjective To clarify the correlations between CD64 expression levels on the surface of peripheral blood lymphocytes and neutrophils and the levels of HBsAg,HBV-DNA and ALT by observing the changes of CD64 expression levels on the surface of peripheral blood lymphocytes and neutrophils of patients with chronic hepatitis B(CHB)before and after interferon treatment in order to provide reference for further study on whether the CD64 on the surface of peripheral blood lymphocytes and neutrophils can be used to evaluate the effect of interferon therapy.Methords Fifty-seven CHB patients were collected,including 33 in the e antigen-positive group and 24 in the e antigen-negative group.All patients were treated with interferon for 48 weeks.Another 16 healthy subjects in the same period were selected as the control group.Flow cytometry was used to detect CD64 on the surface of peripheral blood lymphocytes and neutrophils.Before treatment with interferon,the expression levels of CD64 on the surface of peripheral blood lymphocytes and neutrophils were compared at 8,12,24 and 48 weeks of treatment.Pearson correlation was used to analyze the correlation between CD64 expression levels and HBsAg,HBV-DNA and ALT levels on peripheral blood lymphocytes and neutrophils of CHB patients treated with interferon at different time.Results Before interferon treatment,the expression of CD64 on the surface of peripheral blood lymphocytes and on the surface of neutrophils in patients with CHB was higher than that in the control group(P<0.05).Compared with the control group,the expression of CD64 on the surface of lymphocytes in the e antigen-positive group and e antigen-negative group was higher;no significant difference was found between e antigen-positive group and e antigen-negative group(P>0.05).Compared with the control group,the expression of CD64 on the surface of neutrophils was higher in the e antigen-positive group,and that in the e antigen-positive group was higher than that in the e antigen-negative group(P<0.05).After interferon

关 键 词：免疫球蛋白Fc gamma受体I型 淋巴细胞 中性粒细胞 慢性乙型肝炎 干扰素-Α 

分 类 号：R4[医药卫生—临床医学]