基于ITS和形态学系统分析新疆桦木属种间系统发育关系  

Phylogenetic Relationship Analysis Based on ITS Sequences and Morphological Characteristics for Betula Species in Xinjiang

作　　者：杨梅花[1] 苗江林 厚富霞 王蕾蕾 程锦涛 Yang Meihua;Miao Jianglin;Hou Fuxia;Wang Leilei;Cheng Jintao(College of Agriculture,Shihezi University,Shihezi Xinjiang 832000,China)

机构地区：[1]石河子大学农学院,新疆石河子832000

出　　处：《西南林业大学学报（自然科学）》2023年第1期179-185,共7页Journal of Southwest Forestry University：Natural Sciences

基　　金：石河子大学高层次人才科研启动项目(RCZK2018C04)资助;教育部人文社会科学项目(18YJCZH220)资助。

摘　　要：以新疆桦木属的6个种和其他10种桦木属植物为材料,利用Clustal Omega和MEGA 7.0软件对ITS基因序列进行遗传分析,同时利用SPSS 25.0软件对7个形态性状进行主成分和聚类分析,探讨新疆桦木属植物的系统发育关系。结果表明:在ITS遗传演化分析中,新疆桦木属中的桦木亚组和柴桦亚组遗传距离较近,为0.0047,桦木亚组内部绝对遗传距离为0.0060;在形态性状主成分分析中,特征值大于1的累计贡献率为84.5%,桦木属植物分类性状主要取决于生活型、叶片形状、果苞背部被毛、树皮剥裂及纹路;新疆桦木属植物ITS序列和形态学聚类结果基本一致,盐桦与甸生桦聚为一组,与《中国植物志》中盐桦归属于桦木亚组,而甸生桦属于柴桦亚组的分类存在一定差异,但与《新疆植物志》中两者被划分在柴桦亚组的结论有相似之处。Six species of Betula Linn.in Xinjiang and 10 other species of Betula Linn.were further studied.Internal transcribed spacer(ITS)gene were used to compare and analyze phylogenetic trees with Cluster Omega and MEGA 7.0 software.Meanwhile,principal component analysis and cluster analysis of were carried out by using SPSS 25.0 software based on 7 morphological traits.The results revealed that phylogenetic trees of ITS indicated the genetic distance between betula and subsect.Fruticosae was 0.0047,and the absolute genetic distance within Xinjiang Betula species was 0.0060.In the principal component analysis of morphological traits,the cumulative contribution rate of eigen value greater than 1 was 84.5%.The taxonomic characters of Betula mainly depended on life form,leaf shape,back coat of fruit bud,bark dehiscence and grain.Betula halophila and Betula humilis are clustered together in the respect of molecule,which is different from the classification of B.halophila and B.humilis in flora of China,but similar to the conclusion that B.halophila and B.humilis belong to the same subgroup in flora of Xinjiang.

关 键 词：桦木属 ITS 形态学 遗传分析 新疆 

分 类 号：S792.15[农业科学—林木遗传育种]