基于PCR的中华蜜蜂囊状幼虫病毒检测技术研究进展  

Progress in PCR-based Techniques for Detection of Chiese Sacbrood Virus

作　　者：曹旭梅 张串联 席芳贵 韩兵庚 张猛 娄文 夏晓翠 CAO Xu-mei;ZHANG Chuan-lian;XI Fang-gui;HAN Bing-geng;ZHANG Meng;LOU Wen;XIA Xiao-cui(Apiculture Institute of Jiangxi Province,Nanchang 330052,China)

机构地区：[1]江西省养蜂研究所,江西南昌330052

出　　处：《蜜蜂杂志》2023年第2期17-22,共6页Journal of Bee

摘　　要：中华蜜蜂囊状幼虫病毒是中华蜜蜂囊状幼虫病的病原,属单正链小RNA病毒,是危害中华蜜蜂最主要的病毒之一。快速、高效、特异性强、灵敏度高的检测方式对遇制中华蜜蜂囊状幼虫病的发生与传播非常重要。对常用的基于PCR的中华蜜蜂囊状幼虫病毒检测方法进行了综述,并对它们的优缺点进行了比较,以期为中华蜜蜂囊状幼虫病毒的检测方法提供参考。Chinese esacbroodvirusisthe pathogen of Chinese sacbrood disease.It is a sin-gle positive-strand small RNA virus and one of the most important viruses that endanger Apis cerana cerana.Rapid,efficient,specific and sen-sitive detection methods are very important to curb the occurrence and spread of the Chinese sacbrood disease.In this paper,the commonly used PCR-based detection methods of Chinese sacbrood virus were reviewed,and their advantages and dis-advantages were compared,in order to provide reference for the detection methods of Chinese sacbrood virus.

关 键 词：中华蜜蜂 中华蜜蜂囊状幼虫病 聚合酶链式反应 

分 类 号：S895[农业科学—特种经济动物饲养]