DC细胞在辐射损伤抗原递呈及T细胞活化中的作用  

Antigen presentation and T cell activation by dendritic cells in radiation damage

作　　者：李倩 耿爽 鄢成名 郭浩鑫 王志鑫 王美玉 刘奔波 王旭 王易龙 杨陟华 朱茂祥[1,2,3] LI Qian;GENG Shuang;YAN Chengming;GUO Haoxin;WANG Zhixin;WANG Meiyu;LIU Benbo;WANG Xu;WANG Yilong;YANG Zhihua;ZHU Maoxiang(School of Public Health,University of South China,Hengyang 421001 China;Institute of Radiation Medicine,Academy of Military Medicine,Academy of Military Sciences,Beijing Key Laboratory of Radiobiology,Beijing 100850 China;College of Life Sciences,Hebei University,Baoding 071002 China)

机构地区：[1]南华大学公共卫生学院,湖南衡阳421001 [2]军事科学院军事医学研究院辐射医学研究所,北京市放射生物学重点实验室,北京100850 [3]河北大学生命科学学院,河北保定071002

出　　处：《中国辐射卫生》2022年第6期657-662,668,共7页Chinese Journal of Radiological Health

基　　金：国家自然科学基金(81673095)。

摘　　要：目的利用体外细胞共培养技术模拟体内肺组织微环境,探索树突状细胞(DC)在辐射损伤细胞的抗原提呈作用。方法^(60)Coγ射线照射的小鼠肺上皮细胞(MLE-12)与骨髓来源DC和/或脾T淋巴细胞培养48 h,流式细胞术检测DC细胞共刺激分子CD80/86和抗原肽识别复合物MHCⅠ/Ⅱ表达水平,T细胞活化标志CD69/28/152表达水平以及CD4^(+)和CD8^(+)亚群细胞数。结果^(60)Coγ射线照射的MLE-12细胞凋亡率呈剂量依赖性增高,明显刺激DC细胞CD80/86和MHC II表达,但对T细胞无直接活化作用;6 Gy照射的MLE-12细胞与DC细胞和T淋巴细胞共培养48 h,T细胞CD69和CD28表达增加,CD4^(+)和CD8^(+)亚群细胞数均明显高于对照组,同时DC细胞出现CD86和MHCI特异性高表达。结论辐射损伤细胞可刺激DC细胞抗原提呈功能,并对T细胞进行活化。Objective To explore dendritic cells(DCs)-mediated antigen presentation for radiation-injured cells by using the in vitro cell co-culture technology to simulate the in vivo microenvironment of the lung tissue.Methods^(60)Coγ-irradiated mouse lung epithelial cells(MLE-12)were cultured with bone marrow-derived DCs and/or splenic T lymphocytes for48 hours.Flow cytometry was used to measure the expression levels of costimulatory molecules(CD80/86)and antigenic peptide recognition complexes(the major histocompatibility complex[MHC]classⅠ/Ⅱ)on DCs and T cell activation markers(CD69/28/152)as well as the numbers of CD4^(+)and CD8^(+)T cells.Results^(60)Coγirradiation significantly increased the apoptosis rate of MLE-12 cells in a dose-dependent manner,and significantly stimulated the expression of CD80/86 and MHCⅡon DCs,without direct activation of T cells.Afterγ(6 Gy)-irradiated MLE-12 cells were co-cultured with DCs and T lymphocytes for 48 h,there were significant increases in the expression of CD69 and CD28 on T cells,the numbers of CD4^(+)and CD8^(+)T cells,and the expression of CD86 and MHC I on DCs,as compared with the control groups.Conclusion Radiation-injured cells can stimulate antigen presentation by DCs and activate T cells.

关 键 词：电离辐射 肺上皮细胞 树突状细胞 T细胞 抗原提呈 

分 类 号：Q691[生物学—生物物理学]