响应面优化伏毛铁棒锤总生物碱微波辅助提取工艺  

Optimization of Microwave-assisted Extraction of Total Alkaloids from Aconitum Flavum Hand.-Mazz. by Response Surface Methodology

作　　者：多杰措 李彩霞[1,2] 许显莉 宋文珠 马世震[1,2] Duo Jiecuo;Li Caixia;Xu Xianli;Song Wenzhu;Ma Shizhen(Northwest Institute of Plateau Biology,Chinese Academy of Sciences,Xining 810008,China;Qinghai Key Lab.of Qinghai-Tibet Plateau Biological Resources,Xining 810008,China;University of Chinese Academy of Sciences,Beijing 100049,China)

机构地区：[1]中国科学院西北高原生物研究所,青海西宁810008 [2]青海省青藏高原特色生物资源研究重点实验室,青海西宁810008 [3]中国科学院大学,北京100049

出　　处：《中国野生植物资源》2023年第1期40-46,共7页Chinese Wild Plant Resources

基　　金：中国科学院青海省人民政府三江源国家公园联合研究专项项目(LHZX-2020-09)。

摘　　要：目的:优化微波辅助提取伏毛铁棒锤总生物碱工艺,并比较不同产地伏毛铁棒锤中总生物碱含量。方法:以单因素试验为基础,结合响应面法,以总生物碱的含量为评价指标,考察温度、料液比、时间和体积分数对提取得率的影响。结果:最佳微波辅助提取工艺参数(条件)为:温度48℃,料液比1∶19(g/mL),时间12 min,甲醇体积分数39%。结论:该提取方法稳定可靠,操作简便,可用于优化伏毛铁棒锤总生物碱提取工艺。比较不同地区伏毛铁棒锤中总生物碱含量,贵南县伏毛铁棒锤总生物碱含量最高,为0.290%。Objective:To optimize the process of microwave-assisted extraction(MAE) of total alkaloids from Aconitum flavum Hand.-Mazz.(A. flavum), and compare the content of total alkaloids in A. flavum from different regions.Methods:Based on the single factor test, combined with the response surface method, the effects of temperature, solid-liquid ratio, time and volume fraction on the extraction yield were investigated by taking the content of total alkaloids as the evaluation index.Results:The optimal MAE process parameters(conditions) were as follows: temperature 48℃, solid-liquid ratio 1:19(g/mL), time 12 min, methanol volume fraction 39%.Conclusion:The extraction method is stable, reliable, and easy to operate, and can be used to the extraction of total alkaloids from A. flavum. Comparing the total alkaloid content of A. flavum in different regions, the total alkaloid content of A. flavum in Guinan County is the highest, which is 0.290%.

关 键 词：响应面法 伏毛铁棒锤 总生物碱 微波辅助提取 

分 类 号：R284.2[医药卫生—中药学]