甘蓝型油菜发育进程中赤霉素动态变化及其与产量的关系  

Dynamic Changes of Gibberellin Content During the Development and Its Relationship with Yield of Brassica napus L.

作　　者：谢伶俐 韦丁一[1,2] 章子爽 徐劲松 张学昆 许本波 XIE LingLi;WEI DingYi;ZHANG ZiShuang;XU JinSong;ZHANG XueKun;XU BenBo(Engineering Research Center of Ecology and Agricultural Use of Wetland,Ministry of Education,Yangtze University,Jingzhou 434025,Hubei;College of Life Science,Yangtze University,Jingzhou 434025,Hubei;College of Agriculture,Yangtze University,Jingzhou 434025,Hubei)

机构地区：[1]长江大学湿地生态与农业利用教育部工程研究中心,湖北荆州434025 [2]长江大学生命科学学院,湖北荆州434025 [3]长江大学农学院,湖北荆州434025

出　　处：《中国农业科学》2022年第24期4793-4807,I0001,共16页Scientia Agricultura Sinica

基　　金：农业农村部项目(15214011);湖北省农业厅项目(鄂农油[2021]9号)。

摘　　要：【目的】中国甘蓝型油菜品系与欧洲甘蓝型油菜品系存在较大的遗传差异,二者组配的杂种F_(1)产量优势明显。测定不同来源的甘蓝型油菜发育进程中GA的动态变化,研究GA含量与亲本及杂种F_(1)产量的关系,分析亲本及杂种后代GA合成途径关键酶基因的转录水平,为探明GA对甘蓝型油菜生长发育的影响及其在产量形成过程中的作用奠定基础。【方法】2020年11月至2021年5月,利用高效液相色谱法测定15个不同来源的中国甘蓝型油菜品系及15个欧洲甘蓝型油菜品系GA含量的动态变化;2021年11月至2022年5月,以杂种F_(1)产量优势较强的YG2009×YC4、ZS11×YC4及其亲本YG2009、ZS11、YC4为材料,考察3个不同时期(D1:2022年1月15日;D2:2022年2月15日;D3:2022年3月15日)亲本及杂种F_(1)的GA含量变化、生长指标(株高、根长和鲜重等)、产量及构成因子(单株角果数、角粒数和千粒重)和光合指标(光合速率、气孔导度、蒸腾速率和胞间CO_(2)浓度),分析GA含量随温度变化的趋势,GA含量与亲本及杂种F_(1)的农艺性状、光合特性和产量的关系,并利用实时荧光定量PCR技术,分析亲本及杂种F_(1)不同时期(D1、D2和D3)GA合成途径关键酶基因的转录水平变化。【结果】GA总量与环境温度密切相关,供试甘蓝型油菜GA含量随温度的变化均呈先降低后升高的趋势,但来源于欧洲的甘蓝型油菜品系平均GA含量高于中国品系。F_(1)产量表现为明显的优势,YG2009×YC4产量比其父、母本分别提高18.06%和10.35%;ZS11×YC4产量比其父、母本分别提高29.92%和28.6%。亲本和杂种F_(1)的部分农艺性状差异显著,产量与GA含量存在相关性。亲本及杂种F_(1)中GA20ox4、GA3ox2及SLR1转录水平的变化较大。【结论】来源于欧洲的甘蓝型油菜品系GA总量高于中国油菜品系,而且对温度变化更加敏感;欧洲油菜和中国油菜品系杂交能产生产量杂种优势,其杂种优�【Objective】It was found that there were significant genetic differences between Chinese and European Brassica napus lines, and the yield advantage of hybrid F_(1) was obvious. To explore the effect of gibberellin(GA) on the growth and development of B. napus, the dynamic changes of GA during the developmental process of B. napus from different lines were measured. For explaining the role of GA in the formation of yield, the transcriptional levels of key enzyme genes involved in GA synthesis were analyzed, and the relationship between GA content and yield in parents and F_(1) was clarified. 【Method】The dynamic changes of GA content in B. napus lines(15 Chinese lines and 15 European lines) with different sources from November 2020 to May 2021were determined using high performance liquid chromatography. Two F_(1)hybrid lines(YG2009×YC4, ZS11×YC4) with strong heterosis and their parents were used as materials to investigate the changes of GA content in different periods(D1, 15 January 2022;D2, 15February 2022;D3, 15 March 2022)and measure growth indicators(plant height, root length, fresh weight, etc.), yield and component factors(silique number per plant, seed number per silique and thousand-seed weight), as well as photosynthetic indicators(photosynthetic rate, stomatal conductance, transpiration rate and intercellular CO_(2) concentration).The change trend of GA content with temperature and the relationship between GA content and agronomic characters, photosynthetic characteristics and yield of parents and F_(1)hybrid from November 2021 to May 2022 were analyzed. Besides, the transcriptional levels of key enzyme genes involved in GA synthesis of the parents and F_(1) at different stages(D1, D2, D3) also examined by quantitative real-time PCR.【Result】The content of GA was closely related to the ambient temperature, and the GA content in the tested B. napus lines decreased first and then increased with the change of temperature. However, it was found that the average content of GA in B. napus lines fro

关 键 词：甘蓝型油菜 杂种优势 赤霉素 产量 转录水平 

分 类 号：S565.4[农业科学—作物学]