玉米发芽期响应盐胁迫的转录组分析  

Transcriptome Analysis of Maize in Response to Salt Stress at Germination Stage

作　　者：王星哲 武悦[2] 王艺煊 王瑞莲[2] 周文新[1] 李瑞莲[1] 陈阳 Wang Xingzhe;Wu Yue;Wang Yixuan;Wang Ruilian;Zhou Wenxin;Li Ruilian;Chen Yang(College of Agronomy,Hunan Agricultural University,Changsha,410000;Agricultural Biotechnology Research Center,Bayannur Academy of Agricultural and Animal Sciences,Bayannur,015000)

机构地区：[1]湖南农业大学农学院,长沙410000 [2]巴彦淖尔市农牧业科学研究院,农业生物技术研究中心,巴彦淖尔015000

出　　处：《分子植物育种》2023年第2期370-378,共9页Molecular Plant Breeding

基　　金：内蒙古自治区科技成果转化专项(BZC191033);内蒙古农牧业科学院青年创新基金项目(2020QNJJNO15);玉米密植高产宜机收品种筛选及其配套栽培技术项目(2016YFD0300308);湖南省光学农业工程技术研究中心项目(2018TP2003)共同资助。

摘　　要：玉米种子发芽期对盐分较为敏感,为了进一步明确玉米发芽期对盐胁迫的应答机制,本研究利用高通量转录组测序技术,对玉米耐盐自交系昌7-2在150 mmol/L NaCl胁迫下的胚芽进行转录组测序和数据分析。结果表明:共有40290个Unigenes获得功能注释,其中差异表达基因615个。在差异表达基因的GO富集分析中,对富集程度明显的前20个类别做出功能分析。有21个DEGs富集到苯丙烷类的生物合成途径,是KEGG富集分析最显著的通路。对差异表达基因进行COG分类,共得到25个不同的COG功能注释。通过对目标基因的挖掘共得到13个较重要的转录因子,涉及到MYB、WRKY、AP2、bZIP、bHLH和NAC等6个家族。本研究为挖掘玉米耐盐相关基因、解析玉米响应盐胁迫的分子调控机制提供科学依据。Maize is more sensitive to salt at germination stage.In order to further clarify the mechanism of maize response to salt stress at germination stage,high-throughput transcriptomic sequencing technology was used for transcriptomic sequencing and data analysis of maize salt-tolerant inbred line Chang7-2 germ under 150 mmol/L NaCl stress.The results showed that a total of 40290 Unigenes were annotated,including 615 DEGs.In the GO enrichment analysis of DEGs,the top 20 classes with obvious enrichment were analyzed.21 DEGs were enriched in phenylpropanoid biosynthesis pathway,which was the most significant KEGG enrichment pathway.The DEGs were classified into 25 COG groups.In addition,13 important transcriptional factor were selected for further analysis,including MYB,WRKY,AP2,bZIP,bHLH and NAC families.This study provided a basis for identification of salt-tolerance related genes and analysis of the molecular regulatory mechanism of maize in response to salt stress.

关 键 词：玉米 盐胁迫 发芽期 转录组 

分 类 号：S513[农业科学—作物学]