兴安落叶松纤维素合酶基因的克隆及生物信息学分析  

Cloning of and Bioinformatics Analysis of Cellulose Synthase(CesA)Genes from Larix gmelinii

作　　者：特日格勒 徐坤 林晓飞[2] 白玉娥[1] Terigele;Xu Kun;Lin Xiaofei;Bai Yu'e(College of Forestry,Inner Mongolia Agricultural University,Hohhot,010019;School of Life Sciences,Inner Mongolia University,Hohhot,010021)

机构地区：[1]内蒙古农业大学林学院,呼和浩特010019 [2]内蒙古大学生命科学学院,呼和浩特010021

出　　处：《分子植物育种》2023年第2期454-463,共10页Molecular Plant Breeding

基　　金：国家自然科学基金项目(31660213,31660212)资助。

摘　　要：纤维素合成酶(cellulose synthase,CesA)是植物体纤维素合成途径中的关键酶,其表达风度影响纤维素的合成及植物的生长发育。纤维素合成酶是由多基因编码,该基因家族的每一个成员有不同的表达模式,可能发挥不同的作用。本研究利用兴安落叶松(Larix gmelinii)的转录组数据,调查了其纤维素合成酶基因家族。在兴安落叶松的转录组数据库中检索到6条编码纤维素合酶的Unigene,根据这些基因序列,利用RACE和RT-PCR技术克隆了其全长基因序列,分别命名为LgCesA1、LgCesA3、LgCesA4、LgCesA5、LgCesA6和LgCesA8,并进行了生物信息学分析。结果发现,该6个LgCesA基因的编码序列长度为829~1100个氨基酸,其编码的蛋白质均定位于质膜上,含有保守的功能结构域,属于跨膜蛋白,它们在进化上可分为两个分支。研究结果为比较分析兴安落叶松CesA基因家族成员的表达模式,鉴定其功能奠定了基础。Cellulose synthase(CesA)is a key enzyme in the pathway of cellulose synthesis,whose expression level affects the synthesis of cellulose and the growth and development of plants.Cellulose synthase is a polygenic family,each member has different expression patterns and may play different roles.In this study,we investigated the cellulose synthase gene family of Larix gmelinii using transcriptome data.The six of unigenes encoding cellulose synthase were retrieved from the transcriptome database and their full-length cDNAs were cloned using RACE and RT-PCR based on these unigene sequences,namely LgCesA1,LgCesA3,LgCesA4,LgCesA5,LgCesA6and LgCesA8 and bioinformatics analysis was performed.The results showed that the six LgCesA genes encode proteins of 829~1100 amino acids which located in plasma membrane with the conserved functional domains.All of the LgCesAs belonged to transmembrane proteins,which could be divided into two branches in evolution.The results lay a foundation for comparatively analyzing the expression patterns and identifying function of CesA gene family members in L.gmelinii.

关 键 词：兴安落叶松(Larix gmelinii) CesA基因 生物信息学分析 

分 类 号：S791.222[农业科学—林木遗传育种]