七叶莲对佐剂性关节炎大鼠的治疗效果及对血清白细胞介素-4、白细胞介素-10和肿瘤坏死因子-α水平的影响  

Therapeutic effect of Schefflera arboricola Hayata on adjuvant arthritis rats and its effect on the levels of serum interleukin-4,interleukin-10 and tumor necrosis factor-α

作　　者：廖婉婷 潘文疆 陈明珠 林少梅 梁荣生 LIAO Wanting;PAN Wenjiang;CHEN Mingzhu;LIN Shaomei;LIANG Rongsheng(Department of Pharmacology,Quanzhou Medical College,Quanzhou 362000,Fujian Province,China;Department of Vascular Intervention,Jinjiang Hospital,Jinjiang 362200,Fujian Province,China)

机构地区：[1]泉州医学高等专科学校药理学教研室,福建泉州362000 [2]晋江市医院血管介入科,福建晋江362200

出　　处：《新乡医学院学报》2023年第2期113-118,共6页Journal of Xinxiang Medical University

基　　金：福建省天然药物药理学重点实验室开放课题(编号:FJNMP-201802)。

摘　　要：目的 探讨七叶莲对佐剂性关节炎(AA)大鼠的治疗效果及其对血清白细胞介素(IL)-4、IL-10、肿瘤坏死因子-α(TNF-α)水平的影响。方法 将50只雄性Sprague Dawley大鼠按随机数字表法分为正常对照组、模型组、吲哚美辛组、低剂量七叶莲组、高剂量七叶莲组,每组10只。模型组、吲哚美辛组、低剂量七叶莲组、高剂量七叶莲组大鼠于左后足趾皮内注射弗氏完全佐剂100μL制备AA模型。造模成功后,吲哚美辛组大鼠给予2.5 mg·kg^(-1)吲哚美辛灌胃作为阳性对照,低剂量七叶莲组和高剂量七叶莲组大鼠分别给予10.0、25.0 mg·kg^(-1)七叶莲灌胃,正常对照组和模型组大鼠给予等体积生理盐水灌胃,均每日1次,连续给药10 d。实验期间观察各组大鼠进食、饮水、皮毛、关节肿胀等一般情况。分别于造模后及给药2、5、8、10 d时,采用关节炎指数(AI)评分评估模型组、吲哚美辛组、低剂量七叶莲组、高剂量七叶莲组大鼠全身关节炎病变程度。分别于造模前、造模后及给药2、5、8、10 d时,使用游标卡尺测量各组大鼠后足跖厚度,以后足跖厚度表示大鼠足跖肿胀度。给药10 d后,采用酶联免疫吸附试验检测各组大鼠血清中IL-4、IL-10、TNF-α水平。结果 实验期间,正常对照组大鼠进食、饮水正常,皮毛整洁,神态活泼,无关节肿胀;模型组大鼠进食、饮水量下降,活动减少,精神倦怠,局部关节肿胀明显,甚至出现溃烂;低剂量七叶莲组和高剂量七叶莲组大鼠给药6 d后,进食、饮水逐渐增加,神态良好,局部出现的溃烂开始结痂;吲哚美辛组上述表现较模型组也有改善。造模后及给药2、5 d时,模型组、吲哚美辛组、低剂量七叶莲组、高剂量七叶莲组大鼠的AI评分比较差异无统计学意义(P>0.05);给药8、10 d时,吲哚美辛组、低剂量七叶莲组、高剂量七叶莲组大鼠AI评分比较差异无统计学意义(P>0.05);给药8、10 d时,�Objective To investigate the therapeutic effect of Schefflera arboricola Hayata on adjuvant arthritis(AA) rats and its effect on serum interleukin(IL)-4,IL-10 and tumor necrosis factor-α(TNF-α) levels.Methods Fifty male Sprague Dawley rats were divided into the normal control group, model group, indomethacin group, low-dose Schefflera arboricola Hayata group and high-dose Schefflera arboricola Hayata group according to random number table method, with 10 rats in each group.Rats in the model group, indomethacin group, low-dose Schefflera arboricola Hayata group and high-dose Schefflera arboricola Hayata group were intradermally injected with 100 μL Freund′s complete adjuvant on the left hind toe to prepare the AA model.After successful modeling constructed, the rats in the indomethacin group were given 2.5 mg·kg^(-1)indomethacin by gavage as a positive control, the rats in the low-dose Schefflera arboricola Hayata group and high-dose Schefflera arboricola Hayata group were given 10 and 25 mg ·kg^(-1)Schefflera arboricola Hayata by gavage, and the rats in the normal control group and model group were given the same volume of normal saline by gavage, once a day for 10 consecutive days.During the experiment, the general conditions of rats in each group were observed, such as eating, drinking, fur, joint swelling, etc.After modeling and 2,5,8,10 days of administration, the degree of systemic arthritis of rats in the model group, indomethacin group, low-dose Schefflera arboricola Hayata group and high-dose Schefflera arboricola Hayata group was evaluated by the arthritis index(AI) score.Before and after modeling, and 2,5,8 and 10 days of administration, the thickness of the hind paw of rats in each group was measured with vernier caliper, and the thickness of the hind paw represented the degree of swelling of the rat′s foot.After 10 days of administration, the levels of serum IL-4,IL-10 and TNF-α of rats in each group were detected by enzyme-linked immunosorbent assay.Results During the experiment, the rats i

关 键 词：七叶莲 佐剂性关节炎 白细胞介素-4 白细胞介素-10 肿瘤坏死因子-Α 

分 类 号：R285.5[医药卫生—中药学]