纳米载体在大鼠深静脉急性血栓治疗中的实验研究  

Experimental study of nanocarriers in the treatment of acute deep vein thrombosis in rats

作　　者：张耀宇 杨满义 牟方志[3] 秦佳军 曹川 于玲霞 官建国[3] 李智 Zhang Yaoyu;Yang Manyi;Mou Fangzhi;Qin Jiajun;Cao Chuan;Yu Lingxia;Guan Jianguo;Li Zhi(School of Medicine,Wuhan University of Science and Technology,Wuhan Hubei,430081;Department of Orthopedics,General Hospital of Central Theater Command of Chinese People's Liberation Army,Wuhan Hubei,430070;State Key Laboratory of Advanced Technology for Material Synthesis and Processing,Wuhan University of Technology,Wuhan Hubei,430070,China)

机构地区：[1]武汉科技大学医学院,湖北武汉430081 [2]中国人民解放军中部战区总医院骨科,湖北武汉430070 [3]武汉理工大学材料复合新技术国家重点实验室,湖北武汉430070

出　　处：《生物骨科材料与临床研究》2023年第1期8-12,共5页Orthopaedic Biomechanics Materials and Clinical Study

基　　金：国家自然科学基金(52073222);湖北省自然科学基金(2019C FA 048)。

摘　　要：目的 探索新型溶栓方式——使用纳米级载体辅助组织性纤溶酶原激活剂(t-PA)进行溶栓,并与t-PA治疗比较溶栓效率。方法 30只SD大鼠分成3组,每组10只,分别为生理盐水组(生理盐水0.1 mL)、单纯溶栓药物组(t-PA 0.1 mL)和载药纳米载体组(载有t-PA的载体)。采用麻醉动物、手术切开、三氯化铁浸润建立大鼠股静脉急性血栓模型,在氯化铁处理20 min后从远端静脉注射药物,治疗时间均为4 h,每间隔1 h使用激光散斑血流成像系统监测股静脉血流状态。绘制每组样本相对血流量变化曲线,并进行统计学分析。结果 生理盐水组、单纯溶栓药物组、载药纳米载体组的血流灌注恢复率分别为-7.80%、21.36%、60.82%,差异具有统计学意义(P=0.016),载药纳米载体组中样本治疗后血流量恢复明显升高。结论 与传统溶栓药物治疗t-PA组相比,载药载体组溶栓效率更高,该载体可在血栓治疗中有良好应用前景。Objective To explore a new way of thrombolysis—use a nanoscale carrier to assist tissue plasminogen activator(t-PA) for thrombolysis, and compare the thrombolysis efficiency with t-PA treatment. Methods Thirty SD rats were divided into 3 groups, namely saline group(saline 0.1 m L), traditional thrombolytic drug group(t-PA 0.1 m L), and nanocarriers group(carrier loaded with t-PA). Each group of 10 rats was used to establish a rat femoral vein thrombosis model by anesthetized animals, surgical incision, and ferric chloride infiltration. After ferric chloride treatment for 20 min,the drug was intravenously injected from the distal end, and the treatment time was 4 hours. The femoral vein blood flow status was monitored by laser speckle blood flow imaging system every hour. Draw the relative blood flow change curve of each group of samples and conduct statistical analysis. Results The blood perfusion recovery rates of the normal saline group, the pure thrombolytic drug group, and nanocarriers group were -7.80%, 21.36%, and 60.82%, respectively, the difference was statistically significant(P=0.016). The blood flow of the samples in nanocarriers group increased significantly after treatment. Conclusion Compared with the traditional thrombolytic drug treatment t-PA group, the thrombolytic efficiency of the nanocarriers group was higher. Thus the nanocarriers show great promise in thrombus treatment.

关 键 词：纳米载体 股静脉血栓 溶栓治疗 动物实验 

分 类 号：R318.08[医药卫生—生物医学工程]