The antioxidant trolox inhibits aging and enhances prostaglandin E-2 secretion in mesenchymal stem cells  

作　　者：XIAOXU ZHANG LIN ZHANG LIN DU HUIYAN SUN XIA ZHAO YANG SUN WEI WANG LISHENG WANG 

机构地区：[1]Laboratory of Molecular Diagnosis and Regenerative Medicine,Medical Research Center,The Affiliated Hospital of Qingdao University,Qingdao,266000,China [2]Department of Hematology,The Affiliated Hospital of Qingdao University,Qingdao,266000,China [3]School of Nursing,Jilin University,Changchun,130000,China [4]Yanda Medical Research Institute,Hebei Yanda Hospital,Sanhe,065201,China

出　　处：《BIOCELL》2023年第2期385-392,共8页生物细胞（英文）

基　　金：supported by the Natural Science Foundation of Shandong Province(ZR2020MH327);Hebei Key Research and Development Program(19272405D);Jilin Scientific and Technological Development Program(Grant No.20190304041YY).

摘　　要：Mesenchymal stem cells(MSCs)have been widely used in regenerative medicine and clinical therapy due to their capabilities of proliferation,differentiation,and immune regulation.However,during in vitro expansion,MSCs are prone to aging,which largely limits their application.Prostaglandin E-2(PGE-2)is a key effector secreted by MSCs to exert immunomodulatory effects.By screening the compound library for PGE-2 secretion,the antioxidant trolox was verified as a stimulator of MSCs to secrete PGE-2.The effect of antioxidant trolox on biological characteristics of MSCS,including aging,proliferation,and gene expression,was examined.The results demonstrated that trolox can resist aging,promote proliferation,and enhance PGE-2 secretion of MSCs without affecting their surface marker expression.Furthermore,trolox treatment up-regulates miR-17-92 clusters in MSCs and may contribute to its anti-aging effects.Thus,trolox addition might be beneficial for MSCs expansion and their application.

关 键 词：Mesenchymal stem cells(MSCs) TROLOX ANTIOXIDATION Prostaglandin E-2 

分 类 号：R95[医药卫生—药学]