Ca^(2+)、Mg^(2+)对硬壳蛤幼虫附着、变态的影响  

Effects of Ca^(2+) and Mg^(2+) on the Attachment Metamorphosis of Larvae of Mercenaria mercenaria

作　　者：石八军 郭永军 李永仁 梁健 梁爽 陈丽梅 马东怡 王瑞 张涛[2] 张中华 黄亚冬 SHI Bajun;GOU Yongjun;LI Yongren;LIANG Jian;LIANG Shuang;CHEN Limei;MA Dongyi;WANG Rui;ZHANG Tao;ZHANG Zhonghua;HUANG Yadong(Tianjin Key Laboratory of Aqua-Ecology and Aquaculture,College of Fishery,Tianjin Agricultural University,Tianjin 300392,China;Institute of Oceanology,Chinese Academy of Sciences,Qingdao 266071,China;Tianjin Haisheng Aquaculture Co.,Ltd.,Tianjin 300200,China)

机构地区：[1]天津农学院水产学院,天津市水产生态与养殖重点实验室,天津300392 [2]中国科学院海洋研究所,青岛266071 [3]天津市海升水产养殖有限公司,天津300200

出　　处：《经济动物学报》2022年第4期293-298,共6页Journal of Economic Animal

基　　金：现代农业产业技术体系专项(CARS-49);天津市重点研发计划项目(20YFZCSN00240);天津市现代农业产业技术体系创新团队建设项目(ITTMRS2021002);天津市农业科技成果转化与推广项目(202103010);天津市高等学校基础科研业务费资助项目(2019KJ041)。

摘　　要：采用在养殖海水中添加不同金属离子诱导物的方法,研究单独添加和混合添加Ca^(2+)、Mg^(2+)对硬壳蛤(Mercenaria mercenaria)眼点幼虫附着和变态的诱导作用,确定了诱导的最佳浓度。结果表明:单独添加和混合添加Ca^(2+)、Mg^(2+)对硬壳蛤幼虫的附着率和变态率均显著高于对照组,其中混合添加Ca^(2+)、Mg^(2+)对硬壳蛤幼虫的附着变态的诱导效果最为显著。当混合添加Ca^(2+)、Mg^(2+)浓度分别为3.125,15.625 mmol/L时,可获得最佳附着率及变态率,分别为85.14%和59.16%;单独添加Ca^(2+)浓度为10 mmol/L,最佳附着率及变态率分别为83.98%和51.18%;单独添加Mg^(2+)20 mmol/L时可获得最佳附着率和变态率,分别为81.04%和45.04%。The induction effects of Ca^(2+)and Mg^(2+)alone and in association on the attachment and metamorphosis of eye-spot larvae of the hard-shelled clam(Mercenaria mercenaria) were studied by adding different metal ion inducers to the cultured seawater, and the optimal concentration for their induction was determined. The results showed that the attachment rate and metamorphosis rate of the larvae of hard-shelled clam by adding Ca^(2+)and Mg^(2+)alone and in association were significantly higher than those of the control group. Among them, the mixed addition of Ca^(2+)and Mg^(2+)had the most significant effect on the induction of attachment metamorphosis of the larvae. When the concentrations of Ca^(2+)and Mg^(2+)were mixed with 3.125 and 15.625 mmol/L, respectively, the best adhesion rate and metamorphosis rate were obtained, which were 85.14% and 59.16%, 83.98% and 51.18%, respectively. The best adhesion rate of 81.04% and metamorphosis rate of 45.04% were obtained when Mg^(2+)20 mmol/L was added alone.

关 键 词：硬壳蛤 附着率 变态率 CA^(2+) Mg^(2+) Ca^(2+)/Mg^(2+) 

分 类 号：S968.31[农业科学—水产养殖]