鸡HSPB1基因多态性及其低氧表达差异分析  

Analysis on Chicken HSPB1 Gene Polymorphisms and Differences in Hypoxic Expression

作　　者：苏握瑜 张莹[1,2] 张浩 张博[1] SU Woyu;ZHANG Ying;ZHANG Hao;ZHANG Bo(National Enginering Laboratory of Livestock and Poultry Breeding,China Agricultural University,Highland Livestock and Poultry Genetic Resources Research Center,Beijing 100193;China Agriculture Museum,Beijing 100026)

机构地区：[1]中国农业大学畜禽育种国家工程实验室,高原畜禽遗传资源研究中心,北京100193 [2]中国农业博物馆,北京100026

出　　处：《中国家禽》2022年第12期1-7,共7页China Poultry

基　　金：现代农业产业技术体系建设专项资金(CARS-40-K05);山东省自然科学基金青年科学基金(ZR2021QC133)

摘　　要：为研究热休克蛋白B1(HSPB1)与藏鸡胚胎低氧适应性的关系,试验对藏鸡和茶花鸡在常氧(O2,21%)和低氧(O2,13%)孵化条件下胚胎尿囊绒毛膜(CAM)和心脏组织HSPB1mRNA表达进行检测以及对HSPB1启动子区活性位点进行筛选验证。结果显示:在鸡胚孵化期第11~16天藏鸡CAM组织中HSPB1基因在低氧环境下的表达显著高于茶花鸡(P<0.05),第14~16天藏鸡心脏组织中HSPB1基因在低氧环境下的表达显著高于茶花鸡(P<0.05)。筛选到位于HSPB1启动子区-593bp处的突变位点,且藏鸡优势基因型TT低氧时转录活性显著高于茶花鸡优势基因型AA(P<0.05)。研究提示HSPB1启动子区含有调控其低氧差异表达的活性位点,且相较于茶花鸡,低氧下藏鸡胚胎尿囊绒毛膜组织和心脏组织HSPB1mRNA的高表达,维持了低氧条件下CAM的血管生成平衡和心脏的正常功能,有利于藏鸡胚胎适应低氧环境。In order to study the relationship between heat shock protein B1(HSPB1) and hypoxia adaptation of Tibetan chicken embryos, HSPB1 gene mRNA expression was detected in Tibetan chicken and Chahua chicken embryos’ membrane(CAM) and heart tissue which incubated under normoxia(O_(2), 21%) and hypoxia(O_(2), 13%), it’s active site of promoter region was screened and verified. The results showed that the expression of HSPB1 gene in the CAM tissue of Tibetan chickens were significantly higher than that of Chahua chickens in the hypoxic environment on the 11th to 16th day of the incubation period of chicken embryos(P<0.05) and the expression of HSPB1 gene in the heart tissue of Tibetan chickens was significantly higher than that of Chahua chickens in the hypoxic environment on the 14th to 16th day of the incubation period of chicken embryos(P<0.05). The mutation sites located in the HSPB1 promoter region-593 bp were screened, and the transcriptional activity of the dominant genotype TT in Tibetan chicken was significantly higher than that of the dominant genotype AA of Chahua chicken(P<0.05). The study suggests that the HSPB1 promoter region contains active sites that regulates its hypoxia differential expression, and compared with Chahua chickens, the high expression of HSPB1 mRNA of CAM and heart tissue in Tibetan chicken under hypoxia maintains the balance of CAM angiogenesis and normal heart function, which help Tibetan chicken embryos adapt to the hypoxic environment.

关 键 词：藏鸡 茶花鸡 HSPB1基因 SNP 低氧适应 

分 类 号：S831[农业科学—畜牧学]