京海黄鸡生长早期骨骼肌可变剪切分析  

Alternative Splicing Analysis of Skeletal Muscle for Jinghai Yellow Chicken During Early Growth

作　　者：车隆 叶琳 吴鹏飞[1] 凌暄泽 张晋 周楷智 王麒帆 张跟喜[1] CHE Long;YE Lin;WU Pengfei;LING Xuanze;ZHANG Jin;ZHOU Kaizhi;WANGQifan;ZHANG Genxi(College of Animal Science and Technology,Yangzhou University,Yangzhou,Jiangsu 225009)

机构地区：[1]扬州大学动物科学与技术学院,江苏扬州225009

出　　处：《中国家禽》2022年第12期21-27,共7页China Poultry

基　　金：江苏省农业重大新品种创制项目(PZCZ201730);现代农业产业技术体系建设专项资金(CARS-41);江苏高校优势学科建设工程(PAPD)

摘　　要：为揭示鸡骨骼肌生长发育过程中关键的可变剪切(Alternative splicing,AS),试验分别采集4(M4)、8(M8)和12(M12)周龄京海黄鸡胸肌用于转录组测序,筛选差异的AS(FDR≤0.05),并进行功能分析。结果显示:在M4 vs M8、M8 vs M12和M4 vs M12比较组中得到786、1 649和1 721条差异AS,分别对应了637、1 239和1 259条来源基因,其中228条为共有基因;GO功能富集分析发现TOP20条目中富集到蛋白质定位调节和细胞内蛋白质转运正调控等多条蛋白相关的生物学过程;KEGG通路分析显著富集到紧密连接和泛素介导的蛋白质水解等通路;利用蛋白互作网络结合GO和KEGG筛选到GAPDH、TRIP12、PDLIM7、CEP290、PTPRC、VTI1B和MYL1等多条关键来源基因。综上可知,可变剪切在鸡骨骼肌发育过程中普遍存在,且不同发育阶段可受相同的AS调控,同时,试验筛选的多条关键基因及其可变剪切在鸡骨骼肌发育过程中发挥重要作用。In order to reveal the key alternative splicing(AS) in the skeletal muscle growth of chicken, breast muscles of Jinghai yellow chickens at 4(M4), 8(M8) and 12(M12) weeks of age were collected for transcriptome sequencing. The alternative splicing with significant difference was screened with FDR≤0.05 and function analysis was conducted. The results showed that 786, 1 649 and 1 721 differentially expressed alternative splicing(DEAS) were found in the M4 vs M8, M8 vs M12 and M4 vs M12 comparison groups. Those DEAS correspond to 637, 1 239 and 1 259 host genes respectively, including 228 common host genes.GO function enrichment analysis was performed for the 228 common host genes. Several biological process(BP) terms related to protein regulation were significantly enriched in top 20, including regulation of protein localization and positive regulation of intracellular protein transport. KEGG pathway analysis showed that endocytosis and ubiquitin mediated proteolysis pathways were significantly enriched. Protein-protein interaction network analysis was performed for the 228 common host genes, and key candidate genes were finally screened combined with GO and KEGG results, such as GAPDH, TRIP12, PDLIM7, CEP290, PTPRC,VTI1B and MYL1. The above results indicated that AS generally exists in the development of chicken skeletal muscle and it was regulated by the same AS at different growth stages. The key candidate genes screened in this experiment and their alternative splicing played an important role in the early development of chicken skeletal muscle.

关 键 词：黄羽肉鸡 转录组测序 可变剪切 骨骼肌 生长发育 

分 类 号：S831[农业科学—畜牧学]