华南忍冬花蕾中1个新的单萜苷类化合物  

A new monoterpene glycoside from Lonicera confusa

作　　者：王团委 王振中[2,3] 王秋红 耿剑亮 WANG Tuan-wei;WANG Zhen-zhong;WANG Qiu-hong;GENG Jian-liang(School of Traditional Chinese Medicine Guangdong Pharmaceutical University,Guangzhou 510006,China;State Key Laboratory of New-tech for Chinese Medicine Pharmaceutical Process,Kanion Pharmaceutical Co.,Ltd.,Lianyungang222001,China;Key Laboratory for the New Technique Research of TCM Extraction and Purification,Kanion Pharmaceutical Co.,Ltd.,Lianyungang 222001,China;Nanjing University of Chinese Medicine,Nanjing 210023,China)

机构地区：[1]广东药科大学中药学院,广东广州510006 [2]江苏康缘药业股份有限公司,中药制药过程新技术国家重点实验室,江苏连云港222001 [3]江苏康缘药业股份有限公司,中药提取精制新技术重点研究室,江苏连云港222001 [4]南京中医药大学,江苏南京210023

出　　处：《中草药》2022年第23期7317-7323,共7页Chinese Traditional and Herbal Drugs

基　　金：国家中医药管理局中央财政转移支付地方项目:基于重点研究室研究领域的中医药多学科研究能力提升项目-中药提取精制新技术。

摘　　要：目的 研究华南忍冬Lonicera confusa干燥花蕾(山银花)的化学成分。方法 综合应用HP-20、硅胶、ODS、Sephadex LH-20和半制备液相等多种色谱学方法进行分离纯化,并根据化合物的理化性质和核磁共振波谱数据进行结构鉴定。采用LPS诱导小鼠巨噬细胞RAW264.7模型,以抑制炎性细胞因子一氧化氮(NO)的分泌为评价指标对分离得到的化合物进行体外抗炎活性评价。结果 从华南忍冬干燥花蕾70%乙醇提取物中分离鉴定了5个化合物,分别为8-[α-L-arabinopyranosyl-(1′′→6′)-β-D-glucopyranosyl]-2,6-dimethyloct-1,2′′-lactone(1)、天师酸(2)、黄麻脂肪酸F(3)、天师酸甲酯(4)和黄麻脂肪酸F甲酯(5)。结论 化合物1为新的单萜苷类化合物,命名为忍冬单萜A,化合物2~5为首次从忍冬属植物中分离得到。化合物对LPS诱导的RAW264.7细胞中NO的分泌均无显著的抑制作用。Objective To study the constituents from the dried flower buds of Lonicera confusa.Methods The compounds were isolated and purified by column chromatography of HP-20 macroporous resin,silica gel,ODS,Sephadex LH-20,and semi-preparative RP-HPLC.Their structures were elucidated by physicochemical properties and spectral analyses.Taking NO as the evaluating indicator,the model of LPS-induced RAW264.7 cells was used to evaluate the in vitro anti-inflammatory activity of isolates.Results Five compounds were isolated and identified from the 70% ethanol extract of L.confusa,including 8-[α-L-arabinopyranosyl-(1′′→6′)-β-D-glucopyranosyl]-2,6-dimethyloct-1,2′′-lactone(1),tianshic acid(2),corchorifatty acid F(3)、tianshic acid methyl ester(4) and corchorifatty acid F methyl ester(5),respectively.Conclusion Compound 1 is a new monoterpene glycoside,named comfusanoside A.Compounds 2—5 are identified from Lonicera for the first time.All the isolates had no significant inhibitory effect on NO secretion in RAW264.7 cells stimulated by LPS.

关 键 词：华南忍冬 山银花 一氧化氮 8-[α-L-arabinopyranosyl-(1″→6′)-β-D-glucopyranosyl]-2 6-dimethyloct-1 2″-lactone 忍冬单萜A 天师酸 黄麻脂肪酸F 天师酸甲酯 

分 类 号：R284.1[医药卫生—中药学]