电针“委中”对腰多裂肌损伤大鼠铁代谢紊乱的保护作用  

Effect of electroacupuncture of“Weizhong”(BL40) on iron metabolism disorder in rats with lumbar multifidus muscle injury

作　　者：吕巧巧 张莉 李霞[2] 田圆 徐菁 陈莉 霍则军[1] Lü Qiao-qiao;ZHANG Li;LI Xia;TIAN Yuan;XU Jing;CHEN Li;HUO Ze-jun(Department of Traditional Chinese Medicine,the Third Hospital of Peking University,Beijing 100191,China;School of Acupuncture-Moxibution and Tuina,Beijing University of Chinese Medicine,Beijing 100029;School of Traditional Chinese Medicine,Hebei University,Shijiazhuang 050031;Department of Rehabilitation Medicine,People's Hospital of Zhejiang Province,Hangzhou 310014;Beijing Byte Beat Network Technology Co,Ltd,Beijing 100086)

机构地区：[1]北京大学第三医院中医科,北京100191 [2]北京中医药大学针灸推拿学院,北京100029 [3]河北大学中医学院,石家庄050031 [4]浙江省人民医院康复医学科,杭州310014 [5]北京字节跳动网络技术有限公司,北京100086

出　　处：《针刺研究》2022年第12期1073-1079,共7页Acupuncture Research

基　　金：国家自然科学基金面上项目(No.81574052)。

摘　　要：目的:观察腰多裂肌损伤后铁代谢紊乱及氧化应激水平,探讨电针“委中”是否通过对铁代谢紊乱的调节作用来调节氧化应激,从而促进损伤腰多裂肌的修复。方法:将SD大鼠随机分为正常组、模型组、电针组,每组6只。采用一次性注射0.5%布比卡因(BPVC)制备腰多裂肌损伤大鼠模型。造模后24 h开始电针双侧“委中”,30 min/次,1次/d,治疗2 d。HE染色法观察腰多裂肌形态变化;普鲁士蓝染色法观察腰多裂肌组织铁小粒数量;Western blot法检测腰多裂肌谷胱甘肽合成酶(GSS)蛋白表达量;荧光定量PCR法检测腰多裂肌铁调节蛋白1(IRP1)、铁转运蛋白(Fpn)、铁蛋白重链1(FTH1)、谷胱甘肽过氧化物酶4(GPX4)mRNA的表达水平;生化法检测腰多裂肌组织谷胱甘肽(GSH)、丙二醛(MDA)含量。结果:与正常组比较,模型组腰多裂肌肌纤维大片坏死、断裂,伴有大量炎性细胞浸润,铁小粒增多,肌纤维间隙增大;电针组肌纤维损伤较模型组减轻,铁小粒减少。与正常组比较,模型组腰多裂肌铁代谢相关指标IRP1 mRNA表达水平升高(P<0.001),Fpn、FTH1 mRNA表达水平降低(P<0.001);与模型组比较,电针组腰多裂肌IRP1 mRNA表达水平降低(P<0.001),Fpn、FTH1 mRNA表达水平升高(P<0.05,P<0.01)。与正常组比较,模型组腰多裂肌氧化应激相关指标GSS蛋白表达量、GPX4 mRNA表达水平、GSH含量降低(P<0.001),MDA含量升高(P<0.001);与模型组比较,电针组腰多裂肌GPX4 mRNA表达水平、GSH含量升高(P<0.05,P<0.01),MDA含量降低(P<0.001)。结论:BPVC致大鼠腰多裂肌损伤后,损伤局部组织出现铁代谢紊乱,电针“委中”可作用于IRP1、Fpn、FTH1,恢复铁代谢平衡,缓解腰多裂肌组织氧化损伤,促进大鼠腰多裂肌损伤后的修复。Objective To observe the effect of electroacupuncture(EA)of“Weizhong”(BL40)on the disorder of iron metabolism and the level of oxidative stress after lumbar multifidus muscle injury(LMMI),so as to explore its mechanisms underlying promoting the repair of LMMI.Methods Male SD rats were randomly divided into normal,model and EA groups(6 rats in each group).The LMMI model was established by injecting 0.5%bupivacaine(BPVC)solution(400μL)into the lumbar multifidus muscle with the syringe-needle close to the spinous process(L4-L5).Twenty-four hours after successful establishment of the model,EA(2 Hz/15 Hz,2 mA)was applied to bilateral BL40 for 30 min,once a day for 2 days.Histopathological changes of the multifid muscle were observed under microscope after H.E.staining,and the iron granules in the multifid muscle tissue observed after Prussian blue staining.The expression of glutathione synthase(GSS)was detected by Western blot,and the expressions of iron regulatory protein 1(IRP1),ferroportin(Fpn),ferritin heavy chain 1(FTH1,iron metabolism-related proteins)and gluta-thione peroxidase 4(GPX4,functions in protecting cells against detrimental lipid peroxidation and governing a novel form of regulated necrotic cell death,called ferroptosis)mRNAs were detected by quantitative real-time PCR.The contents of glutathione(GSH)and malondialdehyde(MDA)were measured by biochemical methods.Results H.E.staining showed large areas of necrosis and breakage of muscle fibers,disordered arrangement of muscle fibers,widened muscle cell space,accompanying with a large number of inflammatory cell infiltration in the multifidus muscle tissue of the model group,which was relatively milder in the EA group.Outcomes of Prussian blue staining showed that compared with the normal group,there were more iron particles in the multifidus muscle tissue and enlarged muscle fiber gaps,which was also milder in the EA group.Compared with the normal group,the expression level of IRP1 mRNA and content of MDA were significantly increased(P<0.001),the ex

关 键 词：多裂肌损伤 电针 铁代谢 氧化应激 

分 类 号：R245.97[医药卫生—针灸推拿学]