电针“中髎”“下髎”对慢传输型便秘大鼠结肠5-羟色胺信号系统及粪便短链脂肪酸的影响  

Effects of electroacupuncture at“Zhongliao”and“Xialiao”on serotonin signaling system and short-chain fatty acids in slow transit constipation rats

作　　者：马嘉泽 陈鹏[1] 张加敏 丁康[2] 周惠芬[2] MA Jia-ze;CHEN Peng;ZHANG Jia-min;DING Kang;ZHOU Hui-fen(Nanjing Hospital of Chinese Medicine Affiliated to Nanjing University of Chinese Medicine,Nanjing 210029,China;Pelvic Floor Center,Nanjing Hospital of Chinese Medicine Affiliated to Nanjing University of Chinese Medicine,Nanjing 210001)

机构地区：[1]南京中医药大学附属南京中医院,南京210029 [2]南京中医药大学附属南京中医院盆底中心,南京210001

出　　处：《针刺研究》2022年第12期1101-1106,1112,共7页Acupuncture Research

基　　金：南京市中医药青年人才项目(No.ZYQ20030);江苏省研究生实践创新计划项目(No.SJCX20_0611)。

摘　　要：目的:观察电针“中髎”“下髎”对慢传输型便秘(STC)大鼠结肠5-羟色胺(5-HT)信号系统及粪便中短链脂肪酸(SCFA)的影响,探讨电针治疗STC的作用机制。方法:SD大鼠随机分为正常组、模型组、阳性药组及电针组,每组8只。采用洛哌丁胺灌胃建立STC大鼠模型。阳性药组予6 g/kg乳果糖灌胃治疗,电针组电针“中髎”“下髎”,每次30 min,两组均每日治疗1次,连续干预14 d。观察各组大鼠首粒黑便排出时间及粪便含水率;Western blot法检测各组大鼠结肠组织中5-羟色胺4受体(5-HT4R)、色氨酸羟化酶1(TPH1)、5-HT转运体(SERT)相对表达量;ELISA法检测各组大鼠血清P物质(SP)、血管活性肠肽(VIP)含量;高效液相色谱-质谱联用法检测各组大鼠结肠组织中5-HT含量及粪便中SCFA含量。结果:与正常组比较,模型组大鼠首粒黑便排出时间延长(P<0.05),粪便含水率降低(P<0.05);结肠组织中5-HT含量及5-HT4R、TPH1、SERT蛋白表达量降低(P<0.05);血清SP含量降低(P<0.05),VIP含量升高(P<0.05);粪便SCFA含量(除异丁酸外)均显著降低(P<0.05)。与模型组比较,电针组和阳性药组大鼠首粒黑便排出时间缩短(P<0.05),粪便含水量升高(P<0.05);结肠组织中5-HT含量及5-HT4R、TPH1、SERT蛋白表达量升高(P<0.05);粪便中乙酸及丁酸含量均明显升高(P<0.05);电针组血清SP含量升高(P<0.05),VIP含量降低(P<0.05)。电针组大鼠血清VIP含量较阳性药组显著降低(P<0.05);结肠组织TPH1、SERT蛋白表达量较阳性药组显著升高(P<0.05)。结论:电针“中髎”“下髎”可以通过干预5-HT信号系统的多个环节促进肠道动力,从而缓解STC。Objective To observe the effect of electroacupuncture(EA)at“Zhongliao”(BL33)and“Xialiao”(BL34)on the 5-hydroxytryptamine(5-HT)signaling system in colon tissue and short-chain fatty acids in feces of rats with slow transit constipation(STC),so as to explore the underlying mechanisms of EA in the treatment of STC.Methods A total of 32 SD rats were randomly divided into normal,model,drug control and EA groups,with 8 rats in each group.The STC model was established by intragastric administration of loperamide for 14 days.The EA stimulation(2 Hz/15 Hz)was performed at bilateral BL33 and BL34 for 30 min,once a day for 14 days.The first black stool de-fecation time and fecal water content were detected after treatment.The expressions of 5-hydroxytryptamine 4 receptor(5-HT4 R),tryptophan hydroxylase 1(TPH1)and 5-HT transporter(SERT)in colon tissues were detected by Western blot.The contents of substance P(SP)and vasoactive intestinal peptide(VIP)in serum were detected by ELISA.The contents of 5-HT in colon tissue and short chain fatty acid(SCFA)in feces were detected by mass spectrometry.Results Compared with the normal group,the fecal water content,the expressions of 5-HT,5-HT4 R,TPH1 and SERT in colon tissue,the content of serum SP were significantly decreased(P<0.05),the first black stool de-fecation time,and the content of serum VIP was significantly increased(P<0.05),the contents of SCFA in feces were significantly decreased except isobutyric acid(P<0.05)in the model group.Compared with the model group,the fecal water content,the expressions of 5-HT,5-HT4 R,TPH1 and SERT in colon tissues,the contents of acetic acid and butyrate in feces were significantly increased(P<0.05)in the EA and drug control groups,the first black stool defecation time was decreased(P<0.05)in the EA and drug control groups,and the content of serum SP was increased and the content of serum VIP was decreased(P<0.05)in the EA group.Compared with the drug control group,the content of serum VIP was significantly decreased(P<0.05),and the e

关 键 词：慢传输型便秘 电针 中髎 下髎 5-羟色胺信号系统 

分 类 号：R245.97[医药卫生—针灸推拿学]