木姜叶柯不同表型叶中二氢查耳酮类物质的差异分析  

Variance Analysis of Dihydrochalcones in Different Phenotypes Leaves of Lithocarpus litseifolius

作　　者：王颂 黄俊彬 徐巧林[1] 陈颖乐 黄文妍 王志宏 余玉娟 曾雷[1] WANG Song;HUANG Junbin;XU Qiaolin;CHEN Yingle;HUANG Wenyan;WANG Zhihong;YU Yujuan;ZENG Lei(Guangdong Provincial Key Laboratory of Silviculture,Protection and Utilization/Guangdong Academy of Forestry,Guangzhou,Guangdong 510520,China)

机构地区：[1]广东省森林培育与保护利用重点实验室/广东省林业科学研究院,广东广州510520

出　　处：《林业与环境科学》2022年第6期159-165,共7页Forestry and Environmental Science

基　　金：广东省林业科技创新项目(2022KJCX012、2021KJCX004、2020KJCX013)。

摘　　要：为分析比较不同表型叶的木姜叶柯Lithocarpus litseifolius在不同月份二氢查耳酮类物质的含量差异,研究通过高效液相色谱法对7、8、9和10月份采摘的大叶型、尖叶型和紫叶型木姜叶柯开展根皮苷、三叶苷和根皮素含量比较分析。结果表明,不同表型叶的木姜叶柯干品中根皮素、根皮苷和三叶苷含量存在显著性差异,其中大叶型木姜叶柯在7月份根皮素含量最高,同样在7月份采集的紫叶型木姜叶柯三叶苷含量最高,达到12.73%,尖叶型木姜叶柯中根皮苷含量整体高于大叶型和紫叶型;另外不同采摘时间对3种有效成分含量亦有明显影响,在7-10月份的采收周期内,三种不同表型叶木姜叶柯中根皮苷含量呈现升高趋势,而且大叶型略低于尖叶型和紫叶型;在7月份,大叶型、尖叶型和紫叶型木姜叶柯中根皮素高于其它月份;就根皮素和三叶苷而言,不同样品在8和9月份采收时,其含量明显低于7月份和10月份样品中的含量。In order to analyze and compare the content differences of dihydrochalcone substances in Lithocarpus litseifolius with different phenotypes leaves, high-performance liquid chromatography was used to analyze the contents of phloridzin, trilobatin and phloretin from L. litseifolius of large-leaf, pointed-leaf and purple leaves picked in July, August, September and October. The results showed that there were significant differences in the contents of phloretin, phlorizin and trilobatin in the different phenotypes leaves. Among them, the content of phloretin in L. litseifolius with large-leaf type was the highest in July. Similarly, the trilobitin content in the purpleleaf type samples collected in July was the highest, reaching 12.73%, and the phloridzin content in the pointedleaf type samples was generally higher than that in the large-leaf type and purple-leaf type. In addition, different picking times also had a significant effect on the content of the three active ingredients. During the picking period from July to October, the content of phloridzin in the three different phenotypes of L. litseifolius showed an increasing trend, the contents of characteristic substances in the large-leaf samples slightly lower than pointed-leaf type and purple-leaf. The contents of phloretin in large-leaf, pointed-leaf and purple-leaf type samples obtained from July were higher than those in other months. As far as phloretin and trilobatin were concerned, the contents of different samples when picked in August and September were significantly lower than those in the samples in July and October. The results of this study have important reference values for clarifying the picking time of leaf samples with different phenotypes and promoting the efficient use of resources.

关 键 词：木姜叶柯 二氢查耳酮 叶表型 采收月份 相关性 

分 类 号：S794.9[农业科学—林木遗传育种]