HPLC指纹图谱和UPLC-Q-TOF-MS方法对芪冬颐心口服液中成分研究  

Qualitative analysis of ingredients in Qidong Yixin Oral Liquid based on HPLC fingerprint and UPLC-Q-TOF-MS method

作　　者：韩美欣 张晨阳 佟红娜 吴海峰[2] 许旭东[2] 于淼 孙桂波[2,3,4] HAN Mei-xin;ZHANG Chen-yang;TONG Hong-na;WU Hai-feng;XU Xu-dong;YU Miao;SUN Gui-bo(Drug Engineering and Technology Research Center,Harbin University of Commerce,Harbin 150076,China;Institute of Medicinal Plant Development,Peking Union Medical College,Chinese Academy of Medical Sciences,Beijing 100193,China;Key Laboratory of New Drug Discovery Based on Classic Chinese Medicine Prescription,Chinese Academy of Medical Sciences,Beijing 100193,China;Key Laboratory for Research and Evaluation of Pharmacovigilance,NMPA,Beijing 100193,China)

机构地区：[1]哈尔滨商业大学药物工程技术研究中心,黑龙江哈尔滨150076 [2]中国医学科学院北京协和医学院药用植物研究所,北京100193 [3]中国医学科学院基于经典名方的新药发现重点实验室,北京100193 [4]国家药品监督管理局“药物警戒研究与评价重点实验室”,北京100193

出　　处：《中草药》2022年第24期7679-7685,共7页Chinese Traditional and Herbal Drugs

基　　金：吉林省科技发展计划项目(20200708039YY);中央支持地方改革发展基金资助项目(2020YQ12);黑龙江省自然科学基金资助项目(YQ2020H024)。

摘　　要：目的建立芪冬颐心口服液的HPLC指纹图谱,结合UPLC-Q-TOF-MS方法对其成分进行归属,为芪冬颐心口服液质量控制提供理论依据。方法建立10批次芪冬颐心口服液指纹图谱,采用“中药指纹图谱相似度评价系统(A版)”进行相似度评价,方法学考察精密度、重复性、稳定性,同时应用UPLC-Q-TOF-MS方法对其成分进行鉴别和归属。结果10批次芪冬颐心口服液指纹图谱中有7个共有峰,指纹图谱相似度大于0.99。同时应用UPLC-Q-TOF-MS方法,正离子质谱数据鉴定出25个成分,负离子质谱数据鉴定出17个成分,正、负离子质谱数据共同鉴定出9个成分,并对每个共有峰进行单味药材归属。结论HPLC指纹图谱结合UPLC-Q-TOF-MS方法准确可靠,重复性和稳定性较好,可为芪冬颐心口服液的质量控制提供参考。Objective The HPLC fingerprint of Qidong Yixin Oral Liquid(芪冬颐心口服液) was established,and its components were assigned by UPLC-Q-TOF-MS method,providing theoretical basis for quality control of Qidong Yixin Oral Liquid.Methods The fingerprint of 10 batches of Qidong Yixin Oral Liquid was established,and the Similarity evaluation system of traditional Chinese medicine fingerprint(Version A) was used to evaluate the accuracy,repeatability and stability of the methodology.At the same time,the UPLC-Q-TOF-MS method was used to identify and classify its components.Results There were 7 common peaks in the fingerprint of 10 batches of Qidong Yixin Oral Liquid,and the similarity of fingerprint of 10 batches of Qidong Yixin Oral Liquid was greater than 0.99.At the same time,UPLC-Q-TOF-MS method was used to identify 25 components from positive ion mass spectrometry data,17 components from negative ion mass spectrometry data,and 9 components from positive and negative ion mass spectrometry data,and each common peak was classified as a single medicinal material.Conclusion HPLC fingerprint combined with UPLC-Q-TOF-MS method is accurate,reliable,reproducible and stable,which can provide reference for quality control of Qidong Yixin Oral Liquid.

关 键 词：芪冬颐心口服液 指纹图谱 UPLC-Q-TOF-MS 毛蕊异黄酮 甲基麦冬黄烷酮A 甘露三糖 地黄苷D 

分 类 号：R284.1[医药卫生—中药学]