EFFECTS OF NAAG AND MPEP ON RAT CORTICAL SPREADING DEPRESSION  

作　　者：ZHEN WANG WEIHUA LUO XIAOLI SUN PENGCHENG LI SHANGBIN CHEN QINGMING LUO 

机构地区：[1]Britton Chance Center for Biomedical Photonics Wuhan National Laboratory for Optoelectronics Huazhong University of Science and Technology Wuhan 430074,P.R.China

出　　处：《Journal of Innovative Optical Health Sciences》2010年第2期123-129,共7页创新光学健康科学杂志（英文）

基　　金：This work is supported by the National High Technology Research and Development Program of China(Grant No.2007AA02Z303);the National Natural Science Foundation of China(Grant No.30970964,30801482,30800313);the Program for New Century Excellent Talents in University(Grant No.NCET-08-0213);the Ph.D.Programs Foundation of Ministry of Education of China(Grant No.20070487058,20090142110054).

摘　　要：Cortical spreading depression(CSD)is a pathophysiological phenomenon.There are sufficient evidences to prove that CSD plays an important role in some neurological disorders.However,exact mechanisms of its initiation and propagation are still unclear.Previous studies showed that glutamate receptors could be concerned with CSD,but those studies were mostly performed oriented to ionotropic glutamate receptors(iGluRs).There is relatively little report about effects of metabotropic glutamate receptors(mGluRs)on CSD.Here,we applied optical intrinsic signal imaging(OISI)combined with direct current(DC)potential recording to examine influences of some mGluRs antagonist(or agonist)on CSD propagation in rat’s brain,to indirectly validate actions of some mGluRs on CSD.We found that N-acetyl-l-aspartyl-l-glutamate(NAAG,an agonist at mGluR3)inhibited the propagation of CSD,and the inhibition was gradually developed with time.However,6-methyl-2-phenylethynyl-pyridine(MPEP,an antagonist of mGluR5)did not produce any significant alterations with the CSD propagation.Our findings suggest that mGluR3 could play an important role in the CSD propagation,but the activity of mGluR5 was comparatively weak.These findings can help to understand the propagation mechanism of CSD,and consider the therapy of some neurological diseases involved with CSD.

关 键 词：Metabotropic glutamate receptors cortical spreading depression optical intrinsic signal imaging direct current potential RATS 

分 类 号：R33[医药卫生—人体生理学]