QUANTITATIVE CONTROL OF OPTICAL CLEARING EFFECTS STUDIED WITH TISSUE-LIKE PHANTOM  

作　　者：JINGYING JIANG WEI CHEN QILIANG GONG KEXIN XU 

机构地区：[1]Department of Biomedical Engineering,College of Precision Instruments and Opto-Electronics Engineering,Tianjin University Tianjin 300072,China [2]State Key Laboratory of Precision Measuring Technology and Instruments Tianjin University,Tianjin 300072,China

出　　处：《Journal of Innovative Optical Health Sciences》2010年第3期195-202,共8页创新光学健康科学杂志（英文）

基　　金：supported by the National Natural Science Foundation of China(NSFC,Nos.30600126,30700168 and 30900275).

摘　　要：Tissue optical clearing by use of optical clearing agents(OCAs)has been proven to have potential to reduce the highly scattering effect of biological tissues in optical techniques.However,the difference in tissue samples could lead to unreliable results,making it difficult to quantitatively control the dose of OCAs during the course of tissue optical clearing.In this work,in order to study the effects of optical clearing,we customized tissue-like phantoms with optical properties of some biological tissue.Diffuse reflectance and total transmittance of tissue-like phantoms with different OCAs(DMSO or glycerol)and porcine skin tissues were measured.Then optical property parameters were calculated by inverse adding-doubling(IAD)algorithm.Results showed that OCAs could lead to a reduction in scattering of tissue-like phantoms as it did to porcine skin tissue in vitro.Furthermore,a series of relational expressions could be fit to quantitatively describe the relationship between the doses of OCAs and the reduction of scattering effects.Therefore,proper tissue-like phantom could facilitate optical clearing to be used in quantitative control of tissue optical properties,and further promote the application potential of optical clearing to light-based noninvasive diagnostic and therapeutic techniques.

关 键 词：Optical clearing effects tissue-like phantom quantitative control optical properties scattering effects optical clearing agents(OCAs) 

分 类 号：TN2[电子电信—物理电子学]