QUANTIFYING THE SHORT LIFETIME WITH TCSPC-FLIM:FIRST MOMENT VERSUS FITTING METHODS  

作　　者：LINGLING XU ZHONG-CHAO WEI SHAOQUN ZENG ZHEN-LIHU ANG 

机构地区：[1]Britton Chance Center for Biomedical Photonics Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and Technology Wuhan 430074,P.R.China [2]Key Laboratory of Biomedical Photonics of Ministry of Education Department of Biomedical Engineering Huazhong University of Science and Technology Wuhan 430074,P.R.China [3]Laboratory of Nanophotonic Functional Materials and Devices South China Normal University Guangzhou,510006,P.R.China

出　　处：《Journal of Innovative Optical Health Sciences》2013年第4期1-10,共10页创新光学健康科学杂志（英文）

基　　金：supported by National Basic Research Program of China(Grant No.2011CB910401);the Science Fund for Creative Research Group of China(Grant No.61121004);the National Natural Sci-ence Foundation of China(Grant Nos.30970691 and 61275059);the Scientific Research Foundation for the Returned Overseas Chinese Scholars,State Education Ministry of China,and the Program for New Century Excellent Talents in University of China(Grant No.NCET-10-0407).

摘　　要：Combing the time corelated single photon counting(TCSPC)with fuorescence lifetime imaging microscopy(FLIM)provides promising opportunities in revealing important information on the microenvironment of cells and tissues,but the applications are thus far mainly limited by the accuracy and precision of the TCSPC-FLIM technique.Here we present a comprehensive in-vestigation on the perforance of two data analysis methods,the first moment(M_(1))method and the conventional least squares(Fitting)method,in quantifying fuorescence lifetime.We found that the Mp method is more superior than the Fitting method when the lifetime is short(70-400ps)or the signal intensity is weak(<10^(3) photons).

关 键 词：Fluorescence lifetimne imaging tim-correlated single photon counting the distribution of the first moment(M_(1)) 

分 类 号：O57[理学—粒子物理与原子核物理]