3D fluorescence emission difference microscopy based on spatial light modulator  

作　　者：Guangyuan Zhao Zihao Rong Cuifang Kuang Cheng Zheng Xu Liu 

机构地区：[1]State Key Laboratory of Modern Optical Instrumentation College of Optical Science and Engineering Zhejiang University,Hangzhou 310027,P.R.China

出　　处：《Journal of Innovative Optical Health Sciences》2016年第3期83-92,共10页创新光学健康科学杂志（英文）

基　　金：This work was financially supported by grants from the National Basic Research Program of China (973 Program)(No.2015CB352003);the National Natural Science Foundation of China (Nos.61377013,61335003,61378051,and 61427818);NSFC of Zhejiang province LR16F050001,Innovation Joint Research Center for iCPS (2015XZZX005-01);Open Foundation of the State Key Laboratory of Modern Optical Instrumentation.

摘　　要：We report three-dimensional fluorescence emission difference(3D-FED)microscopy using a spatial light modulator(SLM).Zero phase,0–2
vortex phase and binary 0-pi phase are loaded on the SLM to generate the corresponding solid,doughnut and z-axis hollow excitation spot,respectively.Our technique achieves super-resolved image by subtracting three di®erently acquired images with proper subtractive factors.Detailed theoretical analysis and simulation tests are proceeded to testify the performance of 3D-FED.Also,the improvement of lateral and axial resolution is demonstrated by imaging 100 nm°uorescent beads.The experiment yields lateral resolution of 140 nm and axial resolution of approximate 380 nm.

关 键 词：SUPER-RESOLUTION fluorescence imaging 3D-FED microscopy spatial light modulator 

分 类 号：TN9[电子电信—信息与通信工程]