孟鲁司特钠联合小儿咳喘灵颗粒对咳嗽变异性哮喘患儿外周血mDCs和pDCs及疗效的影响  

Impact of montelukast sodium combined with Xiaoerkechuanling granules on peripheral blood mDCs and pDCs,and clinical effect in children with cough variant asthma

作　　者：陶钧宇 袁晓云 TAO Junyu;YUAN Xiaoyun(Department of Pediatrics,Dongxihu District Maternal and Child Health Hospital,Wuhan 430040,Hubei,China;Department of Pediatrics,Hankou Hospital District of Hubei Central War Zone General Hospital,Wuhan 430010,Hubei,China)

机构地区：[1]东西湖区妇幼保健院儿科,湖北武汉430040 [2]湖北省武汉市中部战区总医院汉口院区儿科,湖北武汉430010

出　　处：《贵州医科大学学报》2023年第1期119-124,共6页Journal of Guizhou Medical University

摘　　要：目的探讨孟鲁司特钠联合小儿咳喘灵颗粒治疗对咳嗽变异性哮喘患儿外周血髓样树突状细胞(mDCs)和浆细胞样树突状细胞(pDCs)及疗效的影响。方法110例咳嗽变异性哮喘患儿随机均分为对照组(常规雾化及孟鲁斯特纳治疗)和观察组(对照组基础上联合小儿咳喘灵颗粒治疗),收集2组患儿的一般临床资料,分别于治疗前和治疗后第1、2、3及4周采集2组患儿外周血静脉血2 mL,采用流式细胞仪四色分析法方法检测外周血mDCs和pDCs,并计算mDCs/pDCs的比值;分别于治疗前、治疗第4周,采用自制病情严重程度量表评估患儿日间和晚间咳嗽严重程度、采用肺功能检测仪检测用力肺活量(FVC)、第1s用力呼气容积(FEV1)/FVC;随访1年,记录2组患儿的复发情况。结果治疗后第1、2、3及4周,2组患儿外周血pDCs比例分别低于治疗前(P<0.05),mDCs/pDCs比值高于治疗前(P<0.05);治疗后第2和3周观察组患儿外周血pDCs比例均低于对照组(P<0.05),外周血pDCs比例高于同期对照组(P<0.05);2组患儿治疗第4周早、晚咳嗽评分低于治疗前(P<0.05),肺功能指标均高于治疗前(P<0.05),且观察组与对照组差异有统计学意义(P<0.05);随访1年后,病例无脱落,观察组复发率(5.45%)低于对照组(18.82%,P<0.05)。结论孟鲁斯特纳联合小儿咳喘灵颗粒治疗可快速降低咳嗽变异性哮喘患儿外周血pDCs比例,提升mDCs/pDCs比值,减轻患儿的临床症状,优化肺功能且预后较好。Objective To investigate the impact of montelukast sodium combined with Xiaoerkechuanling granules on peripheral blood myeloid dendritic cells(mDCs)and plasmacytoid dendritic cells(pDCs),and clinical effect in children with cough variant asthma.Methods A total of 110 children with cough variant asthma were randomly divided into control group(conventional atomization plus montelukast sodium)and observation group(conventional atomization plus montelukast sodium+Xiaoerkechuanling granules).General clinical information of two groups were documented.Two mililiters of peripheral venous blood were collected before treatment and at 1 week,2 weeks,3 weeks,and 4 weeks after treatment.mDCs and pDCs in peripheral blood were detected by flow cytometry through four color staining,and the ratio of mDCs to pDCs was calculated.Before treatment and at the 4thweek after treatment,self-made disease severity scale was used to evaluate the severity of daytime and nighttime cough,and forced expiratory volume in the first second(FEV1)and forced vital capacity(FVC)were measured using a pulmonary function detector.These children were followed for one year.Results At the 1st,2nd,3rd,and 4thweeks after treatment,the proportions of pDCs in peripheral blood of the 2 groups were lower than those before treatment(P<0.05),and the ratios of mDCs/pDCs were higher than those before treatment(P<0.05).The proportions of pDCs in peripheral blood of observation group was lower than those of control group at the 2ndand 3rdweeks after treatment(P<0.05),but higher than those of control group at the same time(P<0.05).At the 4thweek of treatment,the scores of morning and night cough in 2 groups were lower than those before treatment(P<0.05),and pulmonary function indexes were higher than those before treatment(P<0.05).There were significant differences between observation and control groups(P<0.05).After one year of follow-up,all patients were followed.In observation versus control groups,the recurrence rate was 5.45%versus 18.82%(P<0.05).Conclusion Montelu

关 键 词：哮喘 咳嗽变异性哮喘 髓样树突状细胞 浆细胞样树突状细胞 孟鲁司特钠 小儿咳喘灵颗粒 临床症状 肺功能 

分 类 号：R246.4[医药卫生—针灸推拿学]