环介导等温扩增技术在2例动物鼠疫疫情快速诊断的应用  

Application of loop mediated isothermal amplification technology in the rapid diagnosis of two animal plague epidemics

作　　者：谢辉[1] 祁芝珍[1] 赵海红[1] 刘中凯 熊浩明[1] XIE Hui;QI Zhi-zheng;ZHAO Hai-hong;LIU Zhong-kai;XIONG Hao-ming(Qinghai insititute for Endemic Disease Prevention and Control,Xining 811602,China)

机构地区：[1]青海省地方病预防控制所,西宁811602

出　　处：《医学动物防制》2022年第12期1159-1161,共3页Journal of Medical Pest Control

基　　金：国家卫生健康委员会鼠疫防治研究重点实验室(共建)(青海省地方病预防控制所)(2019PT310004)。

摘　　要：目的检验环介导等温扩增技术(loop mediated isothermal amplification,LAMP)在动物疫情中应用效果。方法分别用LAMP、细菌培养、鼠疫耶尔森菌反向血凝试验、鼠疫胶体金免疫层析法4种方法对疫源地2例动物鼠疫疫情的脏器材料进行检测。结果LAMP与细菌培养、鼠疫耶尔森菌反向血凝试验、鼠疫胶体金免疫层析法3种方法检测结果一致,该方法在65℃保温1 h可实现鼠疫耶尔森菌快速检测。结论LAMP法首次直接用于新鲜动物脏器(脾、肝、胸腔积液、肺)和腐败动物材料(骨髓)的检测,而无需细菌分离和DNA提取,具有敏感度高、特异性强、快速简便的特点,可用于鼠疫现场快速检测。Objective To test the application effect of loop mediated isothermal amplification(LAMP)in animal epidemic.Methods Use LAMP,bacterial culture,Yersinia pestis reverse blood coagulation test(RIHA)and plague colloidal gold immunochromatography(GICA)methods to detect the organ materials of 2 cases of animal plague epicenter.Results The coincidence rate of LAMP and bacterial culture,RIHA and RGICA methods were consistent.This method can achieve rapid detection of Yersinia pestis by incubating at 65°C for 1 hour.Conclusions The LAMP method is directly used for the first time for the detection of fresh animal organ materials(spleen,liver,pleural effusion,lung)and spoiled animal materials(bone marrow)without the need for bacterial isolation and DNA extraction.It has high sensitivity,strong specificity,fast and simple features.The method can be used for rapid detection of plague epidemic site.

关 键 词：环介导等温扩增 鼠疫耶尔森菌 动物疫情 快速诊断 现场应用 腐败材料 

分 类 号：R378.61[医药卫生—病原生物学] R516.8[医药卫生—基础医学]