猪细小病毒非结构蛋白NS1通过NF-κB信号通路介导炎症反应  

Porcine parvovirus nonstructural protein NS1 inducing inflammatory through NF-κB signal pathway

作　　者：徐盟龙 阮静娴 王栋涵 王平利[1] 王林青[2] 夏璐 XU Menglong;RUAN Jingxian;WANG Donghan;WANG Pingli;WANG Linqing;XIA Lu(College of Veterinary Medicine,Henan Agricultural University,Zhengzhou 450046,China;Zhengzhou Normal University,Zhengzhou 450044,China)

机构地区：[1]河南农业大学动物医学院,河南郑州450046 [2]郑州师范学院,河南郑州450044

出　　处：《中国兽医学报》2022年第12期2356-2362,共7页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目(31772722);河南省高等学校重点科研基金资助项目(18A230001)。

摘　　要：为了探究猪细小病毒(porcine parvovirus,PPV)非结构蛋白NS1介导炎症反应的情况,将PPV NS1重组质粒转染至PK-15细胞,应用实时荧光定量PCR和免疫印迹技术分别检测炎性因子IL-6/TNF-α的表达和NF-κB信号通路相关蛋白的激活。结果显示,PPV NS1极显著诱导IL-6和TNF-α mRNA的表达(P<0.01)。PPV NS1可显著促进IκBα的降解、p65的磷酸化来激活NF-κB信号通路(0.01<P<0.05)。此外,PPV NS1还促进了Toll样受体2(toll-like receptor 2,TLR2)的表达,TLR2的激动剂和抑制剂分别极显著激活或抑制PPV NS1诱导的IL-6和TNF-α的表达(P<0.01)。结果表明,PPV NS1通过TLR2激活NF-κB信号通路上调炎性细胞因子IL-6和TNF-α的表达。To investigate the activation of inflammatory response by the non-structural protein NS1 of porcine parvovirus(PPV),PPV NS1 recombinant plasmid was transfected into PK-15 cells.Real-time quantitative PCR and Western blot were used to detect the expression of inflammatory factor and NF-κB pathway-related protein.Our findings demonstrated that PPV NS1 could induce the mRNA expression of IL-6 and TNF-α(P<0.01).In addition,PPV NS1 promoted the degradation of IκBα and phosphorylation of p65(0.01<P<0.05).PPV NS1 also promoted the expression of toll-like receptor 2(TLR2).At the same time,the agonist and inhibitor of TLR2 showed TLR2 plays an important role in the activation of NF-kB signal pathway inducing by PPV NS1(P<0.01).In conclusion,these results demonstrate that PPV NS1 induces up-regulation of inflammatory cytokines via TLR2-activated NF-B signaling pathway.These findings provide a new way to study the innate immune mechanism of PPV infection.

关 键 词：猪细小病毒 NS1 TLR2 NF-ΚB 炎性因子 

分 类 号：S852.65[农业科学—基础兽医学]