新城疫、禽流感和禽腺病毒病三联嵌合型病毒样颗粒的构建及鉴定  

Construction and identification of the ND-AI-FAdV4 chimeric virus like particles

作　　者：冯嘉轩 陈凯楠 李金斗 丁佳欣 陈铭桦 邵亚男 郭春红 邹映雪 丁壮 FENG Jiaxuan;CHEN Kainan;LI Jindou;DING Jiaxin;CHEN Minghua;SHAO Yanan;GUOChunhong;ZOU Yingxue;DING Zhuang(Key Laboratory for Zoonosis Research of Ministry of Education,College of Veterinay Medicine,Jilin University,Changchun 130062,China;College of Clinical Medicine,Changchun University of Traditional Chinese Medicine,Changchun 130117,China)

机构地区：[1]吉林大学动物医学学院人兽共患病研究教育部重点实验室,吉林长春130062 [2]长春中医药大学临床医学院,吉林长春130117

出　　处：《中国兽医学报》2022年第12期2368-2373,2400,共7页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目(32072860);吉林省科技厅重点研发计划资助项目(20200402037NC);长春市科技局重点研发计划资助项目(21ZGN17)。

摘　　要：采用新城疫病毒样颗粒载体平台和昆虫杆状病毒表达系统,将禽流感H9N2株HA、禽4型腺病毒Fiber2嵌合至新城疫病毒样颗粒载体表面进而构建“新流腺”三联嵌合型病毒样颗粒(ND-AI-FAdV4 cVLPs)。PCR及免疫荧光检测结果显示,成功构建了重组杆状病毒rBV-cFiber2;透射电镜结果显示,ND-AI-FAdV4 cVLPs直径约为100 nm、含有囊膜的空心蛋白颗粒;免疫印迹结果显示,嵌合型病毒样颗粒各组分蛋白均正确表达。本试验为新城疫、禽流感和禽腺病毒病的安全、高效病毒样颗粒新型疫苗候选株的应用奠定了基础。Based on NDV-like particle vector platform and insect baculovirus expression system,the HA of avian influenza H9 N2 strain and Fiber2 of avian adenovirus type 4 were chimeric on the surface of ND VLPs to construct ND-AI-FAdV4 cVLPs.The results of PCR and immunofluorescence showed that the recombinant baculoviruses rBV-cFiber2 were successfully constructed.Transmission electron microscopy(TEM)showed that ND-AI-FAdV4 cVLPs were encapsulated hollow protein particles with a size of about 100 nm.The results of western blotting showed that the chimeric virus-like particles were all correctly expressed.This study laid the foundation for the application of safe and efficient virus-like particle vaccine candidates against Newcastle disease,avian influenza and avian adenovirus disease.

关 键 词：新城疫 禽流感 禽腺病毒病 病毒样颗粒 昆虫杆状病毒表达系统 

分 类 号：S852.65[农业科学—基础兽医学]