转移因子联合La Sota株免疫对NDV F_(48)E_(9)株强毒重复感染鸡的保护作用  

Protective effect of transfer factor combined with La Sota strain on chicken repeatedly infected with NDV F_(48)E_(9) strain

作　　者：徐磊[1,2] 余勋信 廖惠珍[3,4] 刘毅发 钟云钦 蒋铃 谢杼倢 闫丽萍 曾亮明 杜君 宋素泉 张渊魁[6] 黄瑜 XU Lei;YU Xunxin;LIAO Huizhen;LIU Yifa;ZHONG Yunqin;JIANG Ling;XIE Zhujie;YAN Liping;ZENG Liangming;DU Jun;SONG Suquan;ZHANG Yuankui;HUANG Yu(Fujian Agricultural Vocational Technical College,Fuzhou 350119,China;Present(Fuzhou)Co.,Ltd.,Fuzhou 350500,China;Fuzhou City Changle District Rural Revitalization and Development Center,Fuzhou 350200,China;Fuzhou Zhongwei Biotechnology Co.,Ltd.,Fuzhou 350012,China;College of Veterinary Medicine,Nanjing Agricultural University,Nanjing 210095,China;Zhaofenghua Biotechnology(Nanjing)Co.,Ltd.,Nanjing 211102,China;Institute of Animal Husbandry and Veterinary Medicine/Fujian Provincial Key Laboratory for Avian Diseases Control and Prevention,Fujian Academy of Agricultural Sciences,Fuzhou 350013,China)

机构地区：[1]福建农业职业技术学院,福建福州350119 [2]派生特(福州)生物科技有限公司,福建福州350500 [3]福州市长乐区乡村振兴发展中心,福建福州350200 [4]福州众为生物科技有限公司,福建福州350012 [5]南京农业大学动物医学院,江苏南京210095 [6]兆丰华生物科技(南京)有限公司,江苏南京211102 [7]福建省农业科学院畜牧兽医研究所、福建省禽病防治重点实验室,福建福州350013

出　　处：《中国兽医学报》2022年第12期2374-2384,共11页Chinese Journal of Veterinary Science

基　　金：福建省第七批引才“百人计划”资助项目;福建省科技计划资助项目(2021S2003);江苏省博士后科研资助计划资助项目(2019K072);福建省高层次人才和青年优秀人才访学研修资助计划资助项目(闽人社文[2019]239号);福建省教育科学“十四五”规划资助项目(FJJKGZ21-104);福州市科技计划资助项目(2020-PT-149);福建省职业院校专业带头人培养计划资助项目(闽教办师[2019]40号);福建省畜禽疫病防控技术重大研发平台资助项目(2014N2003);福建省禽病防治重点实验室开放基金资助项目(KF201902)。

摘　　要：为了解猪脾转移因子(transfer factor,TF)对新城疫病毒(Newcastle disease virus,NDV)La Sota弱毒疫苗株免疫效果的影响,本研究采用10^(5.17)EID_(50)La Sota株单独免疫(单独免疫1组)或与TF联合免疫(联合免疫1组)SPF鸡,14 d后以10^(4.7)ELD_(50)F_(48)E_(9)株首次攻毒,设立对照1组(非免疫/首次攻毒),首次攻毒14 d后以10^(9.1)ELD^(50)F_(48)E_(9)株二次攻毒,设立对照2组(非免疫/非首次攻毒/二次攻毒)和空白1组(非免疫/非攻毒),通过淋巴细胞增殖率测定法、血凝抑制试验、荧光定量RT-PCR方法检测T淋巴细胞转化水平(PHA-P SI)、B淋巴细胞转化水平(LPS SI)、新城疫(ND)抗体效价、病毒血症、排毒与主要组织的F48E9株感染情况。结果显示:首次攻毒后,单独和联合免疫1组保护率均为100%,对照1组病死率为100%;二次攻毒后,单独和联合免疫1组保护率分别为20%和50%、病死率分别为60%和40%,对照2组病死率为100%。免疫后14 d,联合免疫1组的PHA-P SI、LPS SI和抗体效价均极显著高于单独免疫1组(P<0.01)。首次攻毒后7~14 d,联合免疫1组的PHA-P SI、LPS SI和抗体效价均极显著高于单独免疫1组(P<0.01),首次攻毒后单独和联合免疫1组均没有检测出病毒血症、泄殖腔和口咽排毒。二次攻毒后3~7 d,联合免疫1组的PHA-P SI、LPS SI和抗体效价均极显著高于单独免疫1组(P<0.01),二次攻毒后联合免疫1组鸡病毒血症率、病毒血症时间、鸡泄殖腔和口咽排毒时间、排毒鸡比例、鸡肝、脾、肺、肾、法氏囊、盲肠扁桃体、脑等主要组织NDV F_(48)E_(9)株感染阳性率均明显低于单独免疫1组。结果表明,TF可提高La Sota株攻毒保护率,提高La Sota株、NDV F_(48)E_(9)株接种后鸡T、B淋巴细胞转化水平和ND抗体效价,降低攻毒后病毒血症率、病毒血症时间、鸡排毒时间、排毒鸡比例和NDV F_(48)E_(9)株在鸡体内的复制。The aim of this study is to evaluate the effect of pig spleen transfer factor(TF)on the immune efficacy of Newcastle disease virus(NDV)attenuated vaccine La Sota strain.Specific pathogen free chickens were inoculated with 10^(5.17)EID_(50)NDV strain La Sota singlely(single vaccine group 1),NDV strain La Sota combined with TF(co-vaccination group 1),respectively,and challenged with 10^(4.7)ELD_(50)virulent NDV strain F_(48)E_(9)on 14 d post vaccination(dpv)and 10^(9.1)ELD_(50)virulent NDV strain F_(48)E_(9)on 14 d post the first challenge(dpfc).The control group 1(nonimmunized but the first challenged),control group 2(non-immunized and non-the first challenged but the second challenged)and blank group 1(non-immunized and non-challenged)were set up simultaneously.In addition,the T lymphocyte transformation level(PHA-P SI),B lymphocyte transformation level(LPS SI),Newcastle disease(ND)antibody titer,viremia,virus shedding and infection of F_(48)E_(9)strain in main tissues were detected by lymphocyte proliferation assay,hemagglutination inhibition test and fluorescence quantitative RT-PCR.The results showed that after the first challenge,the protection rate of single vaccine group 1 and co-vaccination group 1 was 100% and the mortality of control group 1 was 100%.After the second challenge,the protection rates of single vaccine group 1 and co-vaccination group 1 were 20% and 50%,respectively,and their mortality was 60% and 40%.In the meantime,the mortality of control group 2 was 100%.On 14 dpv,the PHA-P SI,LPS SI and antibody titer in co-vaccination groupl were significantly higher than those in single vaccine group 1(P<0.01).From 7 dpfc to 14 dpfc,the PHA-P SI,LPS SI and antibody titer in co-vaccination group 1 were significantly higher than those in single vaccine group 1(P<0.01).In addition,after the first challenge,the viremia,virus shedding from cloacal and oropharyngeal could not be detected in single vaccine group 1 and co-vaccination group 1.From 3 d post the second challenge(dpsc)to 7 dpsc,the PHA-P SI,LPS S

关 键 词：猪脾转移因子 鸡新城疫病毒 重复感染 攻毒保护率 免疫相关分子 

分 类 号：S852.4[农业科学—基础兽医学] S852.61[农业科学—兽医学]