低盐胁迫后两种龙须菜(Gracilariopsis lemaneiformis)叶绿素荧光特征的对比分析  

Effects of low salinity stress on chlorophyll fluorescence characteristics in two Gracilariopsis lemaneiformis strains

作　　者：李晓梅 杨晓琪 段德麟[1,2] LI Xiao-mei;YANG Xiao-qi;DUAN De-lin(CAS and Shandong Province Key Laboratory of Experimental Marine Biology,Center for Ocean Mega-Science,Institute of Oceanology,Chinese Academy of Sciences,Qingdao 266071,China;Laboratory for Marine Biology and Biotechnology,Pilot National Laboratory for Marine Science and Technology(Qingdao),Qingdao 266237,China;University of Chinese Academy of Sciences,Beijing 100049,China)

机构地区：[1]中国科学院海洋研究所海洋大科学研究中心实验海洋生物学重点实验室,山东青岛266071 [2]青岛海洋科学与技术试点国家实验室海洋生物学与生物技术功能实验室,山东青岛266237 [3]中国科学院大学,北京100049

出　　处：《海洋科学》2022年第12期128-137,共10页Marine Sciences

基　　金：山东省自然科学基金(ZR2019BC024)。

摘　　要：以龙须菜981品种和其绿色突变型为材料,运用叶绿素荧光分析技术,比较低盐胁迫(盐度28和22)下二者光合生理的差异。研究结果证实,在正常状态下,龙须菜绿色突变型具有较高的光能利用效率(α)、最大相对电子传递速率(r_(ETRmax))、半饱和光强(Ik)和光性能指数(PIabs),表明其光合性能优于龙须菜981。在2种低盐条件下培养3d,盐度28处理后,龙须菜981的最大相对电子传递速率下降了20.4%,而龙须菜绿色突变型无显著变化,表明盐度28可使龙须菜981的光合活性减弱。在盐度22培养3 d后,龙须菜981的最大光量子产量(FV/Fm)下降幅度高达16.0%,而龙须菜绿色突变型的下降幅度为11.9%;龙须菜981电子传递的量子产额(φ_(EO))下降了20.3%,而龙须菜绿色突变型则无显著变化;另外,盐度22处理后,龙须菜981的光能利用效率和最大相对电子传递速率分别下降31.5%和22.1%,下降幅度均高于龙须菜绿色突变型,这表明龙须菜绿色突变型光合活性对低盐胁迫的耐受性高于龙须菜981。Chlorophyll fluorescence was used to compare the differences in photosynthetic physiological characteristics between two Gracilariopsis lemaneiformis strains,such as strain 981 and its green mutant,under low salinity stress(28 and 22).The results showed that under normal conditions,the values of light energy use efficiency(α),maximum relative electron transfer rate(rETRmax),half-saturation light intensity(Ik),and the performance index(PIabs)were higher in the mutant than those in strain 981,indicating the higher photosynthetic performance of the mutant strain.After exposure to salinity stress of 28 for three days,the photosynthetic performance of strain 981 decreased significantly,as evidenced by the 20.4% decrease in rETRmax,while no significant change was observed in the mutant.When the two G.lemaneiformis strains were exposed to a salinity of 22 for three days,the values of maximum quantum yield for primary photochemistry(FV/Fm) decreased by 16.0% and 11.9% in strain 981 and the mutant,respectively.The quantum yield for electron transport(φEO) value decreased by 20.3% in strain 981,whereas no significant change was detected in the mutant.The decreases in α and rETRmax in the mutant at a salinity of 22 were lower than those of strain 981,suggesting that the low salinity tolerance of the mutant strain was higher than that of strain 981.In conclusion,our results demonstrate that the green mutant is an ideal resource for improving the G.lemaneiformis germplasm.

关 键 词：龙须菜 低盐胁迫 叶绿素荧光 

分 类 号：Q945.11[生物学—植物学] Q946