OsWD40过表达水稻根系响应盐胁迫的转录组分析  

Transcriptome Analysis of OsWD40 Overexpression Rice Roots in Response to Salt Stress

作　　者：闻丹妮 鲍聆然 刘蒙蒙 沈波[1] Wen Danni;Bao Lingran;Liu Mengmeng;Shen Bo(College of Life and Environmental Sciences,Hangzhou Normal University,Hangzhou 311121,Zhejiang,China)

机构地区：[1]杭州师范大学生命与环境科学学院,浙江杭州311121

出　　处：《作物杂志》2022年第6期42-53,共12页Crops

基　　金：浙江省“十四五”农业新品种选育重大科技专项(2021C02063-6);杭州市农业科研攻关项目(20191203B08)。

摘　　要：盐胁迫是影响水稻产量的主要因素之一,开展水稻耐盐机制的研究十分必要。为了揭示Os WD40基因参与耐盐的分子机制,以日本晴和Os WD40过表达水稻株系为材料,用浓度为200mmol/L的Na Cl分别处理0、12、24和48h,对其根系进行转录组测序分析。结果显示,比较日本晴和Os WD40过表达株系在盐胁迫相同时间(ST0与NT0、ST12与NT12、ST24与NT24、ST48与NT48)的基因表达量,分别检测到1950、1646、3499和1522个差异表达基因。其中,盐胁迫处理24h的差异表达基因多于0、12和48h处理。对4个比较组的差异表达基因分别进行GO功能富集分析和KEGG代谢通路分析,发现差异表达基因主要富集在盐胁迫响应、脱落酸响应和转录调控等GO条目中,富集的重要代谢通路主要是植物激素信号转导,植物MAPK信号传导途径和苯丙烷生物合成、类黄酮生物合成相关的次生代谢途径等。同时,转录因子家族基因,如WRKY、MYB和bHLH等,在各比较组中呈现差异表达。由此推测,苯丙烷生物合成和类黄酮生物合成等植物次生代谢途径在Os WD40过表达水稻根系响应盐胁迫中发挥着重要作用,而且Os WD40可能介导响应脱落酸的基因转录调控,激活下游盐胁迫相关基因的表达。Salt stress is a major factor affecting rice yield. Therefore, it is necessary to investigate salt tolerance mechanisms in rice. In order to reveal the molecular mechanism of OsWD40 gene involved in salt tolerance,comparative transcriptome analysis was performed on roots of Nipponbare and OsWD40 overexpression rice line treated with 200mmol/L NaCl for 0, 12, 24 and 48h, respectively. The results showed that a total of 1950, 1646,3499 and 1522 differentially expressed genes were identified between Nipponbare and OsWD40 overexpression line at the same time of salt stress(ST0 vs NT0, ST12 vs NT12, ST24 vs NT24 and ST48 vs NT48). Among them, the number of differentially expressed genes of 24h was more than those of 0, 12 and 48h treatment. GO functional enrichment analysis and KEGG metabolic pathway analysis were performed on differentially expressed genes in four comparison groups, which found that the differentially expressed genes were mainly enriched in salt stress response, abscisic acid response, transcriptional regulation, and so on. The important metabolic pathways also were enriched, including plant hormone signal transduction, plant MAPK signal transduction pathway, phenylpropane biosynthesis, and secondary metabolic pathways related to flavonoid biosynthesis. Meanwhile, the transcription factors, such as WRKY, MYB and bHLH, were differentially expressed in each comparison group. We speculated that phenylpropane biosynthesis, flavonoid biosynthesis and other plant secondary metabolic pathways play an important role in roots of OsWD40 overexpression rice in response to salt stress, and OsWD40 may mediate the regulation of gene transcription in response to abscisic acid and activate the expression of downstream salt stress related genes.

关 键 词：水稻 盐胁迫 转基因 转录组分析 

分 类 号：S511[农业科学—作物学]