甜菜种质资源苗期耐旱性综合评价  

Comprehensive Evaluation of Drought Tolerance of Sugar Beet Germplasms at Seedling Stage

作　　者：李王胜 王雪倩 尹希龙 石杨 刘大丽[1,2] 谭文勃 兴旺[1,2] Li Wangsheng;Wang Xueqian;Yin Xilong;Shi Yang;Liu Dali;Tan Wenbo;Xing Wang(National Medium-Term Repository of Sugar Beet Germplasm,Heilongjiang University,Harbin 150080,Heilongjiang,China;College of Advanced Agriculture and Ecological Environment,Heilongjiang University,Harbin 150080,Heilongjiang,China)

机构地区：[1]黑龙江大学国家甜菜种质中期库,黑龙江哈尔滨150080 [2]黑龙江大学现代农业与生态环境学院,黑龙江哈尔滨150080

出　　处：《作物杂志》2022年第6期54-60,共7页Crops

基　　金：国家作物种质资源库“甜菜分库运行服务”(NCGRC-2021-017);农业农村部“甜菜种质资的收集、鉴定、编目、繁种与入库(圃)保存”(1921-26);国家糖料产业技术体系项目(甜菜种质资源鉴定与新种质创制)(CARS-170102);黑龙江省自然科学基金项目(LH2019C057);黑龙江省高校基本科研业务费黑龙江大学专项资金项目(KJCX201920)。

摘　　要：对336份甜菜种质资源苗期的15个表型和生理生化指标进行测定,并通过隶属函数、主成分分析、聚类分析和相关性分析方法对甜菜种质资源耐旱性进行综合评价。结果表明,干旱胁迫后叶干重、株高、根长、叶鲜重、根鲜重、根干重、叶片饱和鲜重和叶片相对含水量等指标均显著降低,根冠比、可溶性糖、可溶性蛋白和脯氨酸含量等4个指标均显著高于对照。主成分分析将15个单一指标转化为6个综合指标,可代表原始数据信息的75.95%。聚类分析将336份甜菜种质资源分为5个类群,其中耐旱性强种质16份,耐旱性较强种质49份,耐旱性中等种质109份,耐旱性较弱种质79份,耐旱性弱种质83份。相关性分析结果显示,胚轴直径、株高、根长、叶鲜重、根鲜重、根干重、叶干重、叶片饱和鲜重、叶片相对含水量和根冠比与D值呈显著相关性。In this study, 336 sugar beet germplasm resources were measured at the seedling stage along with 15phenotypic, physiological and biochemical indices, and the drought tolerance of these resources was thoroughly assessed using the affiliation function method, principal component analysis, cluster analysis and correlation analysis. The results showed that the indicators of leaf dry weight, plant height, root length, leaf fresh weight,root fresh weight, root dry weight, leaf saturated fresh weight and leaf relative water content were significantly decreased, and root to crown ratio, soluble sugar content, soluble protein content and proline content under drought stress were significantly higher than the control. The principal component analysis transformed the 15single indicators into six composite indicators that could represent 75.95% of the original data information.Cluster analysis divided the 336 sugar beet germplasm resources into five groups, including 16 germplasms with strong drought tolerance, 49 with relatively strong drought tolerance, 109 with medium drought tolerance, 79with relatively weak drought tolerance and 83 with weak drought tolerance. Correlation analysis showed that embryonic axis diameter, plant height, root length, leaf fresh weight, root fresh weight, root dry weight, leaf dry weight, leaf saturated fresh weight, relative leaf water content and root-shoot ratio were significantly correlated with D values.

关 键 词：甜菜 种质资源 干旱胁迫 综合评价 

分 类 号：S566.3[农业科学—作物学]