The Improvement of Soybean Salt Tolerance by Overexpressed GmPAO1  

作　　者：Yeyao Du Yang Song Ye Zhang Sujie Fan Hanzhu Zhang Piwu Wang 

机构地区：[1]College of Agronomy,Jilin Agricultural University,Changchun,130118,China

出　　处：《Phyton-International Journal of Experimental Botany》2023年第4期1109-1124,共16页国际实验植物学杂志（英文）

基　　金：supported by Jilin Province Science and Technology Development Plan Project,Grant No.20190103120JH;Jilin Province Science and Technology Development Plan-Outstanding Young Talents Fund Project,Grant No.20190103120J;The fourth batch of Jilin Province Youth Science and Technology Talent Support Project,Grant No.QT202020;National Natural Science Foundation of China Projects,Grant No.31801381.

摘　　要：Polyamines play an important regulatory role during plant growth and development and adversity stress,and polyamine oxidase(PAO)is involved in polyamine catabolism.In this study,an up-regulated polyamine oxidase gene GmPAO1 was obtained by transcriptome sequencing analysis and screening at soybean seedling stages.Also,its expression pattern and function were analyzed.The identification results of transgenic GmPAO1 soybean positive lines showed that the relative expression level of GmPAO1 in the overexpressed lines was increased under salt stress.With increasing stress concentration,the seed germination rate decreased.However,the seed germination rate of the overexpressed lines was significantly higher than that of the control lines,and the phenotypic character of the root systems was also better than that of the control lines.The measurement of superoxide dismutase(SOD)and peroxidase(POD)activities and malondialdehyde and hydrogen peroxide contents revealed that the overexpressed soybean lines significantly increased the SOD and POD activities,significantly reducing the malondialdehyde content.Although the hydrogen peroxide content in the transformed plants gradually increased,the hydrogen peroxide content in the overexpression lines was still lower than that in the gene editing lines.Based on this,it was preliminarily judged that GmPAO1 can improve soybean salt tolerance.

关 键 词：SOYBEAN polyamine oxidase salt tolerance overexpressed 

分 类 号：S565.1[农业科学—作物学]