Antifungal Potential of Beauveria bassiana on Solanum lycopersicum L. Infected with Fusarium oxysporum f. sp. lycopersici  

作　　者：Henry López-López Nancy Ruiz-Lau Rocío Meza-Gordillo Víctor Manuel Ruiz-Valdiviezo Joseph Galthier Robledo-Luchetti Carlos Alberto Lecona-Guzmán Juan JoséVillalobos-Maldonado Luc Dendooven Joaquín Adolfo Montes-Molina 

机构地区：[1]Laboratorio de Biotecnología vegetal,Tecnológico Nacional de Mexico/Campus Tuxtla Gutiérrez,Tuxtla Gutiérrez,29050,Mexico [2]CONACYT-Tecnológico Nacional de Mexico/Campus Tuxtla Gutiérrez,Tuxtla Gutiérrez,29050,México [3]ABACUS,Cinvestav,Ciudad de México,07360,México

出　　处：《Phyton-International Journal of Experimental Botany》2023年第4期1235-1255,共21页国际实验植物学杂志（英文）

基　　金：funded by the Tecnológico Nacional de México(TECNM):Project No.6602.18-P.

摘　　要：The objective of this work was to evaluate the effect of Beauveria bassiana(Bb 1205)on controlling Fusarium oxysporum f.sp.lycopersici(Fol 17108)in tomato plants in greenhouse conditions.Inoculation of Bb 1205 was the most promising among the agronomic variables and expression of the activity of the enzymesβ-1,3-glucanases and chitinases.Inoculation of Bb 1205 occurred at a concentration of 1×108 conidia·mL−1,which was administered onto the leaves,directly into the soil and via injection.Infection with Fol 17108 occurred with 1×106 spores·mL−1,which were added directly to the soil.Spectrophotometry was used for measuring agronomic parameters,namely activity of chitinases andβ-1,3-glucanases in foliage and roots.When Bb 1205 was added to the soil,the chlorophyll index and aerial part length showed significant differences.In addition,it was determined that root length,fresh weight of foliage,flower,and fruit count increased 82 days after inoculation(dai).Chitinase activity induced by Bb 1205 in leaves and roots of tomato plants infected with Fol 17108 was observed when injected into the stem at 32 dai(41.8 and 11.6-fold,respectively).Inoculation on the foliage showed a 10-fold increase ofβ-1,3-glucanases in the roots after 82 dpi.As for leaves,a 3.8-fold increase was found when the stem was inoculated.In the different in vivo applications,Bb 1205 activated its defenses by expressing the chitinase enzymes andβ-1,3-glucanase,thus reducing the damage caused by Fol 17108,demonstrating increase plant growth thereafter.

关 键 词：MYCOPARASITISM agronomic parameters CHITINASES β-1 3-glucanases 

分 类 号：S641.2[农业科学—蔬菜学]