Identification and Evaluation of Insect and Disease Resistance in Transgenic Cry1Ab13-1 and NPR1 Maize  

作　　者：Yongjing Xi Zhou Yang Yukun Jin Jing Qu Shuyan Guan Siyan Liu Piwu Wang 

机构地区：[1]Joint Laboratory of International Cooperation in Modern Agriculture Technology of Ministry of Education,Plant Biotechnology Center,Jilin Agricultural University,Changchun,China

出　　处：《Phyton-International Journal of Experimental Botany》2023年第4期1257-1274,共18页国际实验植物学杂志（英文）

基　　金：supported by the National Key Research and Development Program of China(2019YFD1002603-1)。

摘　　要：PCR detection,quantitative real-time PCR(q-RTPCR),outdoor insect resistance,and disease resistance identification were carried out for the detection of genetic stability and disease resistance through generations(T2,T3,and T4)in transgenic maize germplasms(S3002 and 349)containing the bivalent genes(insect resistance gene Cry1Ab13-1 and disease resistance gene NPR1)and their corresponding wild type.Results indicated that the target genes Cry1Ab13-1 and NPR1 were successfully transferred into both germplasms through tested generations;q-PCR confirmed the expression of Cry1Ab13-1 and NPR1 genes in roots,stems,and leaves of tested maize plants.In addition,S3002 and 349 bivalent gene-transformed lines exhibited resistance to large leaf spots and corn borer in the field evaluation compared to the wild type.Our study confirmed that Cry1Ab13-1 and NPR1 bivalent genes enhanced the resistance against maize borer and large leaf spot disease and can stably inherit.These findings could be exploited for improving other cultivated maize varieties.

关 键 词：MAIZE NPR1 gene Cry1Ab13-1 gene disease resistance insect resistance 

分 类 号：S513[农业科学—作物学]