Genome-Wide Identification, Evolution and Expression Analyses of GA2ox Gene Family in Brassica napus L.  

作　　者：Yanhua Li Hualei Huang Youming Shi Shuqin Huang Tao Liu Changming Xiao Xiaoqing Tian Ping Zhao Xiaoyan Dai Taocui Huang Yan Zhou 

机构地区：[1]Chongqing Academy of Agricultural Sciences,Chongqing,401329,China [2]Chongqing Municipal Agricultural School,Chongqing,401329,China [3]Fengdu Agricultural Technology Service Center,Chongqing,408201,China

出　　处：《Phyton-International Journal of Experimental Botany》2023年第3期815-835,共21页国际实验植物学杂志（英文）

基　　金：supported by the Chongqing Academy of Agricultural Sciences Youth Innovation Team Project(NKY-2018QC01);Chongqing Finance Special Project(NKY-2022AC002);the Natural Science Foundation Project of Yongchuan(2021yc-jckx20013);the Technology Innovation and Application Development(Surface)Project of Yongchuan(2021yc-cxfz30007);the National Oilseed Rape Industrial Technology System Sanxia Comprehensive Experiment Station Project(CARS-13).

摘　　要：Gibberellin 2-oxidases(GA2ox)are important enzymes that maintain the balance of bioactive GAs in plants.GA2ox genes have been identified and characterized in many plants,but these genes were not investigated in Brassica napus.Here,we identified 31 GA2ox genes in B.napus and 15 of these BnaGA2ox genes were distributed in the A and C subgenomes.Subcellular localization predictions suggested that all BnaGA2ox proteins were localized in the cytoplasm,and gene structure analysis showed that the BnaGA2ox genes contained 2–4 exons.Phylogenetic analysis indicated that BnGA2ox family proteins in monocotyledons and dicotyledons can be divided into four groups,including two C_(19)-GA2ox and two C_(20)-GA2ox clades.Group 4 is a C_(20)-GA2ox Class discovered recently.Most BnaGA2ox genes had a syntenic relationship with AtGA2ox genes.BnaGA2ox genes in the C subgenome had experienced stronger selection pressure than genes in the A subgenome.BnaGA2ox genes were highly expressed in specific tissues such as those involved in growth and development,and most of them were mainly involved in abiotic responses,regulation of phytohormones and growth and development.Our study provided a valuable evolutionary analysis of GA2ox genes in monocotyledons and dicotyledons,as well as an insight into the biological functions of GA2ox family genes in B.napus.

关 键 词：Brassica napus GA2ox gene family EVOLUTION expression patterns 

分 类 号：S565.4[农业科学—作物学]