粪便颗粒大小对大熊猫类固醇激素测定的影响  

Effect of Fecal Particle Size on Fecal Steroids in Giant Panda

作　　者：杨波 唐丹 赵兰兰 杜锴 徐翔 李德红 潘超 李蓉 龚海兵 胡荣强 林冰森 罗波 黄炎 YANG Bo;TANG Dan;ZHAO Lanlan;DU Kai;XU Xiang;LI Dehong;PAN Chao;LI Rong;GONG Haibing;HU Rongqiang;LIN Bingsen;LUO Bo;HUANG Yan(China Conservation and Research Centre for the Giant Panda,Key Laboratory of National Forestry and Grassland Administration on Conservation Biology of Rare Animals in the Giant Panda National Park,Qionglai and Minshan Mountains Conservation Biology of Endangered Wild Animals and Plants National Permanent Scientific Research Base,Dujiangyan,611830,China;Jianyang Municipal Bureau of Planning and Nature Resources,Jianyang,641400,China;Agriculture,Animal Husbandry,Rural Science and Technology Bureau of Danba County,Danba,626300,China)

机构地区：[1]中国大熊猫保护研究中心,大熊猫国家公园珍稀动物保护生物学国家林业和草原局重点实验室,邛崃山、岷山濒危野生动植物保护生物学国家长期科研基地,都江堰611830 [2]简阳市规划和自然资源局林业服务中心,简阳641400 [3]丹巴县农牧农村和科技局,丹巴626300

出　　处：《野生动物学报》2023年第1期172-178,共7页CHINESE JOURNAL OF WILDLIFE

基　　金：大熊猫国际合作资金项目(WL05);中国大熊猫保护研究中心2018科研年项目(CCRCGP181915)。

摘　　要：利用不同孔径筛网对真空冷冻干燥后的大熊猫(Ailuropoda melanoleuca)粪便进行筛取以量化粪便颗粒大小,采用酶联免疫吸附法(ELISA)测定粪便类固醇激素,探究粪便颗粒大小与激素含量的相关性。结果显示:筛网孔径在3个指标下(雌酮-3-葡糖苷酸、孕酮和皮质醇)均存在主效应,且激素含量大致呈现出随筛网孔径增加而减小的特点。3.35 mm(6目)与1.70 mm(10目)孔径筛网筛选的筛下物激素含量整体表现出最低水平,0.88 mm(18目)的激素含量居中,0.43 mm(35目)、0.23 mm(65目)和0.12 mm(120目)的整体水平最高,这3个激素水平组间均存在显著差异性(P<0.05)。此外,由于0.43、0.23、0.12 mm之间激素含量差异不显著,同时考虑到实操性,0.43 mm筛网是较为合适的选择。分别在粪便取样试管的上部、中部与下部取样测量,显示激素含量存在不同程度的差异,表明粪样的充分混合是必要的。本研究提供了一种适用于大熊猫粪便类固醇监测的冻干研筛法和一种可供参考的混样方法,为大熊猫粪便类固醇激素研究提供方法借鉴,以期为野生大熊猫内分泌研究提供技术指导,也为今后大熊猫非损伤性取样研究提供有价值的参考。In this study, vacuum freeze-dried feces of giant panda(Ailuropoda melanoleuca) were screened by mesh screens with different apertures to quantify fecal particle size. Additionally the correlation between fecal steroid content and fecal particle size was determined by enzyme-linked immunosorbent assay(ELISA) in the first time. Results showed that fecal particle size induced differences in estrogen-3-glucuronide(E1G), progesterone(P4) and cortisol(CORT). Fecal steroids content generally increased with the increase of sieve mesh number. Fecal steroids content was the lowest after treatment with 6-mesh(aperture 3. 35 mm) & 10-mesh(aperture 1. 70 mm) sieves, then followed by 18-mesh(aperture 0. 88 mm), and was highest with 35-mesh(aperture 0. 43 mm) & 65-mesh(aperture 0. 23 mm) & 120-mesh(aperture 0. 12 mm). There were significant difference among these three groups(P<0. 05). In addition there was no significant difference among 35-mesh, 65-mesh and 120-mesh, and from the practical prospective, the mesh screen of 35-mesh may be a better choice. We also found that hormones differed in the top, middle and bottom of fecal samples and the need for adequate mixing of fecal samples was explained. Our findings not only provide a method of freeze-drying screening for fecal steroid monitoring in panda, but also provide one referential method of sample mixing. This study provides a technical guidance for wild giant panda endocrinology research, and also provides valuable reference for the future study of giant panda using non-invasive sampling approach.

关 键 词：大熊猫 粪便颗粒大小 类固醇激素 冻干研筛 

分 类 号：Q548[生物学—生物化学]