侵染白附子的芋花叶病毒分子变异研究  

Molecular variability of the dasheen mosaic virus infecting Typhonium giganteum Engl.

作　　者：秦艳红[1] 高素霞[1] 刘玉霞[1] 文艺[1] 杨瑾 李雪梦 王凤丽 戚文平 康宇静 王飞[1] 鲁传涛[1] QIN Yanhong;GAO Suxia;LIU Yuxia;WEN Yi;YANG Jin;LI Xuemeng;WANG Fengli;QI Wenping;KANG Yujing;WANG fei;LU Chuantao(Henan Key Laboratory of Crop Pest Control,Integrated Pest Management Key Laboratory in Southern part of North China for Ministry of Agriculture,Institute of Plant Protec-tion,Henan Academy of Agricultural Sciences,Zhengzhou 450002,China;Kaifeng Academy of Agriculture and Forestry,Kaifeng 475004,China)

机构地区：[1]河南省农业科学院植物保护研究所,河南省农作物病虫害防治重点实验室,农业部华北南部作物有害生物综合治理重点实验室,郑州450002 [2]开封市农林科学研究院,开封475004

出　　处：《植物病理学报》2022年第6期881-890,共10页Acta Phytopathologica Sinica

基　　金：国家中药材产业技术体系(CARS-B21);河南省中药材产业技术体系(Z2019-13-01);河南省农业科学院新兴学科发展专项(2020XK05、2021XK02);河南省农业科学院基本科研业务费项目(2021ZC47)。

摘　　要：为明确侵染白附子的芋花叶病毒(dasheen mosaic virus,DsMV)的分子变异情况,对51个DsMV白附子分离物(DsMV-BF)的外壳蛋白(Coat Protein,CP)基因和3个分离物的近全长基因组序列进行了克隆和测定,DsMV-BF的CP基因大小有855个和942个核苷酸两种类型,51个白附子分离物之间CP基因的核苷酸和氨基酸一致率分别为88.3%~100%和91.9%~100%,BF8、BF30和BF38分离物之间多聚蛋白的核苷酸和氨基酸序列一致率分别为82.9%~95.9%和90.7%~95.9%,与GenBank中其他分离物之间多聚蛋白的核苷酸和氨基酸序列一致率分别为76.9%~99.4%和85.6%~99.0%;P1基因的分子变异较大,P1基因大小有987个和990个核苷酸两种类型;CP基因核苷酸序列系统进化树分析结果表明,侵染白附子的DsMV分离物可分为两个亚组;重组分析结果表明BF8和BF30分离物各检测到1个重组事件,BF38检测到2个重组事件。To elucidate the molecular diversity of dasheen mosaic virus(DsMV)infecting T.giganteum Engl,the coat protein(CP)genes of fifty-one DsMV-BF isolates and near-complete genomic sequences of three isolates were cloned and sequenced.The CP gene of DsMV-BF isolates had the length of 855 bp and 942 bp.Pairwise comparisons of CP gene nucleotide(nt)and amino acid(aa)sequence showed that 51 DsMV-BF isolates had 88.3%-100%nt and 91.9%-100%aa sequence identity with each other.Pairwise comparisons of polyprotein gene sequences showed that BF8,BF30 and BF38 isolates had 82.9%-95.9%nt and 90.7%-95.9%aa sequence identity with each other.Whereas,they displayed 76.9%-99.4%nt and 85.6%-99.0%aa identity with other isolates in the GenBank.P1 gene having lengths of 987 bp or 990 bp,exhibited the highest genetic variability.Phylogenetic tree analysis based on CP gene indicated that DsMV-BF isolates were divided into two subgroups.Recombination analysis showed that BF8 and BF30 isolates were associated with one potential recombination event,respectively.Furthermore,BF38 isolate was detected to have two potential recombination events.

关 键 词：芋花叶病毒 分子变异 重组分析 

分 类 号：S432.41[农业科学—植物病理学]