北京葡萄溃疡病病原菌Lasiodiplodia citricola的鉴定和生物学特性初探  

Identification and biological characteristics of Lasiodiplodia citricola causing Botryosphaeria dieback of grapes in Beijing

作　　者：吴林娜 李兴红[1] 张玮[1] 周悦妍 燕继晔[1] WU Linna;LI Xinghong;ZHANG wei;ZHOU Yueyan;YAN Jiye(Beijing Key Laboratory of Environment Friendly Management on Fruit Disease and Pests in North China,Institute of Plant Protection,Beijing Academy of Agricultural and Forestry Sciences,Beijing 100097,China)

机构地区：[1]北方果树病虫害绿色防控北京市重点实验室,北京市农林科学院植物保护研究所,北京100097

出　　处：《植物病理学报》2022年第6期1003-1005,共3页Acta Phytopathologica Sinica

基　　金：财政部和农业农村部:国家现代农业产业技术体系(CARS-29);北京市农林科学院杰出科学家专项(JKZX201905)。

摘　　要：葡萄溃疡病是由葡萄座腔菌科(Botryosphaeriaceae)真菌引起的,易造成葡萄果梗干枯、果实干缩或掉粒,枝条或枝干顶梢枯死或表面出现溃疡斑,维管束组织可见褐或黑色的不规则坏死斑,进而影响树势,严重时整株干枯死亡^([1-2])。2020年的田间病害调查发现,北京密云区两个葡萄园内葡萄溃疡病发病比往年严重,发病率均超15%,其中某一采样点病株茎表皮肿大开裂(图1-A),整株干枯死亡率为12%,这与之前溃疡病的典型症状不同。Botryosphaeria dieback is one of the important grapevine trunk diseases in the world.In this study,two grapevine trunk disease samples were collected from two grape vineyards in Miyun District of Beijing,where grapevine trunk disease was very severe.The potential pathogens were isolated,purified and identified.Meanwhile this study conducted to evaluate the pathogenicity by Koch’s Postulate,and the effects of temperature and light on the growth of pathogens.According to morphological characteristics and a multi-gene phylogenetic analysis with sequences of ITS,EF-1αand TUB,four isolates were identified as Lasiodiplodia citricola,which was the causal organism of grapevine Botryosphaeria dieback.Moreover,the pathogen was able to grow between 10-40℃.The optimum temperature for mycelial growth was 30℃,and it grew rapidly under full light.This is the first report of grapevine Botryosphaeria dieback disease on grapes caused by L.citricola in China.

关 键 词：葡萄溃疡病 维管束组织 坏死斑 典型症状 溃疡斑 顶梢 葡萄园 生物学特性 

分 类 号：S432.44[农业科学—植物病理学]