百香果疮痂病病原菌鉴定  

Identification of the pathogen causing scab disease on passion fruit(Passiflora Linn.)

作　　者：史国英 曾泉 叶雪莲 马明亮 胡春锦 SHI Guoying;ZENG Quan;YE Xuelian;MA Mingliang;HU Chunjin(Microbiology Research Institute,Guangxi Academy of Agricultural Sciences,Nanning 530007,China;Agricultural and rural Bureau of Xing′an County,Xing′an 541300,China)

机构地区：[1]广西壮族自治区农业科学院微生物研究所,南宁530007 [2]广西兴安县农业农村局,兴安541300

出　　处：《植物病理学报》2022年第6期1006-1008,共3页Acta Phytopathologica Sinica

基　　金：广西自然科学基金(2020GXNSFAA297096);广西科技计划项目(桂科AB18221048);广西农科院基本科研业务专项(桂农科2021JM89,2021YT098)。

摘　　要：百香果产业是广西重点扶贫产业。百香果规模化种植,加之平棚式栽培,在高温高湿季节易暴发真菌性病害。广西百香果常见真菌性病害包括疫病^([1])、炭疽病^([2])、茎基腐病^([3])、果腐病^([4])等。病害问题已成为制约广西百香果产业发展的重要因素之一。2020年10月—2021年5月对广西百香果病害展开调查,先后在南宁市青秀区刘圩镇、岑溪市马路镇的果园发现一种百香果新病害—疮痂病。该病主要为害幼果,初期为褐色凸起小点.To identify the pathogen causing scab disease on the fruit of Passiflora Linn.in the passion fruit yard of Cenxi and Nanning cities,Guangxi province.The scab tissues collected from diseased fruits were isolated,purified,and made pathogenicity test,morphological observation,biological and molecular identification.The representive isolate B31,obtained from the diseased fruit,was selected for morphological observation and pathogen identification,and was verified as the causal agent of the scab disease through Koch’s postulates.The result of morphological observation using light microscope showed that the isolate B31 was very similar to Alternaria in terms of hyphae and spore morphology.Phylogenetic analysis results using multiple gene loci showed that identities of sequences of ITS,Alt al,TEF 1 and endoPG genes of isolate B31 were 100%identical to those of Alternaria alternata.This study clarifies that the pathogen causing scab disease of passion fruit in Guangxi is A.alternata,and this is the first report of A.alternate causing scab on passion fruit in China.

关 键 词：高温高湿季节 百香果 疮痂病 病原菌鉴定 茎基腐病 果腐病 新病害 规模化种植 

分 类 号：S436.68[农业科学—农业昆虫与害虫防治]