香梨树腐烂病菌表型菌株生物学特性及致病性  

Biological characteristics and pathogenicity of phenotypic strains of fragrant pear rot pathogen

作　　者：董力畅 王喆 王兰[1] 沙帅帅 郝海婷 DONG Lichang;WANG Zhe;WANG Lan;SHA Shuaishuai;HAO Haiting(College of Agricultural,Tarim University/Key Laboratory of Integrated Pest Management(IPM)of Xinjiang Production and Construc-tion Corps in Southern Xinjiang/The National-Local Joint Enoineering Laboratory of High Efficiency and Superior-Quality Culti-vatlon and Fruit Deep Processing Iechnology on Characteristic Fruit Trees,Alar 843300,China)

机构地区：[1]塔里木大学农学院/南疆农业有害生物综合治理兵团重点实验室/南疆特色果树高效优质栽培与深加工技术国家地方联合工程实验室,阿拉尔843300

出　　处：《植物病理学报》2022年第6期1013-1017,共5页Acta Phytopathologica Sinica

基　　金：国家自然基金-新疆联合基金重点支持项目(U1903206);旱区作物逆境生物学国家重点实验室开放课题项目(CSBAA2020009);新疆维吾尔自治区研究生科研创新项目(XJ2021G293)。

摘　　要：0引言库尔勒香梨是新疆的特色梨种,近年来由于腐烂病的发生,造成巨大的经济损失^([1]),已成为制约农民增产增收的主要因素。梨树腐烂病菌因产孢体和孢子的形态受环境影响大,故形态鉴定准确性较差,导致国内外病原菌学名使用混乱^([2-6])。本试验从库尔勒香梨主产区采集分离得到303株腐烂病菌菌株,选取菌落表型存在明显差异的8株作为研究对象,明确其种类,测定其生物学特性和致病性分化,为揭示香梨树腐烂病菌遗传多样性提供参考.In order to clarify the intraspecies diversity and pathogenic differentiation of Valsa pyri,eight strains with markedly different colony phenotypes out of the 303 strains isolated from Korla fragrant pear producing area were screened out for biological characteristics and pathogenicity research.According to the difference of colony color and sporulation ability on PDA medium,eight strains were divided into four phenotypes:easy to sporulate with golden yellow,hard to sporulate with cream yellow,brown green and gray black.Biological research results showed that the mycelial growth rate of the four phenotypic strains was the fastest at 28℃,full light,pH 5,wettable starch as carbon source and glutamic acid as nitrogen source,but there were differences among different phenotypes.The results of pathogenicity test showed that there was pathogenicity differentiation between different phenotypes,in which the brown green phenotype strain were classified to the strong pathogenicity type,the gray black and golden yellow phenotype strain was determined to belong to medium pathogenicity type,while the milky yellow phenotype strain was affiliated with weak pathogenicity type.The results provide a basis for revealing the population diversity of pathogen and the control of Korla fragrant pear tree rot.

关 键 词：库尔勒香梨 形态鉴定 腐烂病菌 致病性分化 生物学特性 环境影响 遗传多样性 产孢体 

分 类 号：S432.1[农业科学—植物病理学]