高盐诱导高血压大鼠心肌间质重构及SHIP-1和IL-6的表达研究  

Myocardial interstitial remodeling and expressions of SHIP-1 and IL-6 in high-salt-induced hypertensive rats

作　　者：曾清清 高忠兰[2] 陈务贤[2] 黄荣杰[2] Zeng Qingqing;Gao Zhonglan;Chen Wuxian;Huang Rongjie(Guangxi Medical University,Nanning 530021,China;Department of Cardiovascular Medicine,The First Affiliated Hospital of Guangxi Medical University,Nanning 530021,China)

机构地区：[1]广西医科大学,南宁530021 [2]广西医科大学第一附属医院心血管内科,南宁530021

出　　处：《广西医科大学学报》2023年第1期54-58,共5页Journal of Guangxi Medical University

基　　金：国家自然科学基金资助项目(No.81860087)。

摘　　要：目的:探讨高盐诱导高血压大鼠心肌间质纤维化及SH2结构域的肌醇5-磷酸酶1(SHIP-1)和白介素6(IL-6)的表达。方法:将25只SD雄性大鼠随机分为对照组和模型组,模型组给予高盐饲料(含3%NaCl)喂养12周,对照组给予普通饲料喂养12周。每两周测量大鼠尾动脉收缩压,12周后称取大鼠体重和心脏重量,计算心脏重量指数(心脏重量/体重)。采用苏木精—伊红(HE)染色及Masson染色观察心肌组织病理学改变。分别采用实时荧光定量PCR(RT-qPCR)法和免疫组化法检测SHIP-1和IL-6 mRNA及其蛋白表达。结果:模型组喂养12周后的收缩压和心脏重量指数均高于对照组(P<0.05)。与对照组比较,模型组大鼠心肌组织大量炎性细胞浸润,胶原纤维显著增多,胶原容积分数(CVF)高于对照组(P<0.05)。模型组心肌组织SHIP-1 mRNA及蛋白表达量低于对照组,IL-6 mRNA及蛋白表达量高于对照组(均P<0.05)。结论:高盐诱导高血压大鼠出现心肌间质纤维化重构,同时SHIP-1表达下调,IL-6表达上调。Objective: To investigate myocardial interstitial fibrosis and the expressions of SH2 domain-containing inositol 5-phosphatase 1(SHIP-1) and interleukin-6(IL-6) in high-salt-induced hypertensive rats. Methods:Twenty-five SD male rats were randomly divided into control group and model group. The model group and the control group were fed respectively with high salt diet(containing 3% NaCl) and ordinary diet for 12 weeks. The rat tail artery pressure was measured once every two weeks. After 12 weeks, the rats’ body and heart weight were weighed and the heart mass index(heart weight/body weight) was calculated. Hematoxylin-eosin(HE) staining and Masson staining were used to observe the histopathological changes of myocardium. The mRNA and protein expressions of SHIP-1 and IL-6 were detected by real-time fluorescence quantitative polymerase chain reaction(RT-qPCR) and immunohistochemistry, respectively. Results: Systolic blood pressure and heart mass index in the model group were higher than those in the control group after 12 weeks of feeding(P<0.05). Compared with the control group, the myocardial tissue of rats in the model group was infiltrated by a large number of inflammatory cells, the collagen fiber significantly increased, and the collagen volume fraction(CVF) was higher than that in the control group(P<0.05). The m RNA and protein expression of SHI-1 in the model group was lower than that in the control group while the mRNA and protein expression of IL-6 was higher than that in the control group(all P<0.05). Conclusion: In high-salt-induced hypertensive rats, myocardial interstitial fibrosis remodeling is observed;SHI-1 expression is down-regulated while IL-6 expression is up-regulated.

关 键 词：高盐诱导高血压 SHIP-1 IL-6 心肌间质纤维化 

分 类 号：R544.1[医药卫生—心血管疾病]