Connecting past and present:single-cell lineage tracing  

作　　者：Cheng Chen Yuanxin Liao Guangdun Peng 

机构地区：[1]Center for Cell Lineage and Development,CAS Key Laboratory of Regenerative Biology,Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine,GIBH-HKU Guangdong-Hong Kong Stem Cell and Regenerative Medicine Research Centre,Guangzhou Institutes of Biomedicine and Health,Chinese Academy of Sciences,Guangzhou 510530,China [2]Center for Cell Lineage and Atlas,Bioland Laboratory(Guangzhou Regenerative Medicine and Health Guangdong Laboratory),Guangzhou 510005,China [3]Institute for Stem Cell and Regeneration,Chinese Academy of Sciences,Beijing 100101,China [4]University of Chinese Academy of Sciences,Beijing 100049,China

出　　处：《Protein & Cell》2022年第11期790-807,共18页蛋白质与细胞（英文版）

基　　金：supported in part by National Key R&D Program of China(2018YFA0801402,2018YFA0107200);the Strategic Priority Research Program of the Chinese Academy of Sciences(XDA16020404);Guangdong Basic and Applied Basic Research Foundation(2019B151502054);Frontier Research Program of Bioland Laboratory(Guangzhou Regenerative Medicine and Health Guangdong Laboratory,2018GZR110105013);Jiazi Research Innovative Project of Bioland Laboratory(2019GZR110108001);Science and Technology Planning Project of Guangdong Province(2020B1212060052).

摘　　要：Central to the core principle of cell theory,depicting cells'history,state and fate is a fundamental goal in modern biology.By leveraging clonal analysis and sin-gle-cell RNA-seq technologies,single-cell lineage trac-ing provides new opportunities to interrogate both cell states and lineage histories.During the past few years,many strategies to achieve lineage tracing at single-cell resolution have been developed,and three of them(in-tegration barcodes,polylox barcodes,and CRISPR barcodes)are noteworthy as they are amenable in experimentally tractable systems.Although the above strategies have been demonstrated in animal develop-ment and stem cell research,much care and effort are still required to implement these methods.Here we review the development of single-cell lineage tracing,major characteristics of the cell barcoding strategies,applications,as well as technical considerations and limitations,providing a guide to choose or improve the single-cell barcoding lineage tracing.

关 键 词：single-cell lineage tracing integration barcodes polylox barcodes CRISPR barcodes 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]