温度和浊度对罗丹明B示踪检测的影响及校正  

Influence of temperature and turbidity on Rhodamine B tracer detection and correction

作　　者：郑雪飞 李春[1] 范晓彦[2] 元光[1] 栾晓宁[1] 姚子庆 李凯[1] Zheng Xuefei;Li Chun;Fan Xiaoyan;Yuan Guang;Luan Xiaoning;Yao Ziqing;Li Kai(Optics and Optoelectronics Laboratory of Qingdao,College of Physics and Optoelectronic Engineering,Ocean University of China,Qingdao 266100,China;College of Mathematics and Physics,Qingdao University of Science and Technology,Qingdao 266061,China)

机构地区：[1]中国海洋大学,物理与光电工程学院青岛市光学光电子重点实验室,山东青岛266100 [2]青岛科技大学数理学院,山东青岛266061

出　　处：《红外与激光工程》2022年第12期234-241,共8页Infrared and Laser Engineering

基　　金：国家重点研发计划(SQ2019 YFC140012);国家自然科学基金青年基金(41906163);国家自然科学基金(42076183)。

摘　　要：由于环境污染日益严重,对污染物进行追踪具有必要性,使用示踪剂罗丹明B进行追踪实验是对污染物溯源的一种有效方式,但在使用罗丹明B荧光传感器进行原位污染物追踪检测中,温度浊度等环境因素变化会对测量结果造成影响,因此需对温度和浊度两个主要环境因素进行补偿校正,从而提高罗丹明B检测准确度。利用荧光分光光度计检测不同浓度罗丹明B荧光光谱,并采用偏最小二乘法(PLS)进行光谱数据分析与建立标准曲线。测量并分析了温度10~60℃和浊度0~55NTU范围的罗丹明B荧光光谱,结果表明罗丹明B的荧光强度与温度呈负相关性,与浊度呈正相关性。由于罗丹明B浓度差值变化率与温度和浊度呈良好线性关系,因此利用罗丹明B浓度差值变化率对不同环境进行补偿校正。在温度和浊度补偿校正之后,浓度检测结果的相对误差分别小于0.48%和0.34%,提高了罗丹明B在不同环境中的检测准确度,并对温度和浊度共同影响时的检测结果进行分析,建立共同补偿校正模型。为抑制温度和浊度对罗丹明B原位检测的干扰提供了一种修正思路。Due to the increasingly serious environmental pollution,it is necessary to trace the pollutants.The tracer Rhodamine B is an effective way to trace the source of pollutants.However,in the in-situ pollutant tracking and detection using Rhodamine B fluorescence sensor,the measurement results will be affected by environmental factors such as temperature and turbidity.Therefore,it is important that the accuracy of the in-situ detection of Rhodamine B is improved by compensating and correcting the two main environmental factors,temperature and turbidity.Fluorescence spectra of Rhodamine B with different concentrations were detected by fluorescence spectrophotometer,and partial least squares(PLS)method was used to analyze the spectral data and establish the standard curve.The fluorescence spectra of Rhodamine B were measured and analyzed in the range of temperature from 10℃to 60℃and turbidity from 0 NTU to 55NTU.The results showed that the fluorescence intensity of Rhodamine B was negatively correlated with temperature and positively correlated with turbidity.Since the rate of change of Rhodamine B concentration difference has a good linear relationship with temperature and turbidity,the rate of change of Rhodamine B concentration difference is used for compensation correction in different environments.After temperature and turbidity compensation correction,the relative errors of concentration detection results were less than 0.48%and 0.34%,respectively,which improved the detection accuracy of Rhodamine B in different environments.What’s more,under the influence of temperature and turbidity,the detection results were analyzed,by doing so,a model of common compensation correction was established.It provides a correction method to suppress the interference of temperature and turbidity in-situ detection of Rhodamine B.

关 键 词：荧光光谱 罗丹明B 补偿校正 温度 浊度 

分 类 号：O433.4[机械工程—光学工程]