煤矿复垦区不同恢复年限沙棘人工林土壤真菌群落特征  

Soil Fungal Community Characteristics of Seabuckthorn Plantation with Different Restoration Years in Coal Mine Reclamation Area

作　　者：秦家凤 李阳 刘广全[2] 艾宁[1,2] 刘长海[1] QIN Jia-feng;LI Yang;LIU Guang-quan;AI Ning;LIU Chang-hai(College of Life Science,Yan'an University,Yan'an716000,China;SCIENCE China Institute of Water Resources and Hydropower Rese,Beijing 100038,China;Shenmu Daliuta No.1 Experimental Middle School,Yulin 719300,China)

机构地区：[1]延安大学生命科学学院,陕西延安716000 [2]中国水利水电科学研究院,北京100038 [3]榆林市大柳塔第一实验中学,陕西榆林719300

出　　处：《土壤通报》2022年第6期1413-1420,共8页Chinese Journal of Soil Science

基　　金：国家自然科学基金项目(32060297、31370541);中国水利水电科学研究院R&D项目(SC0145B012021);国家重点研发计划课题(2016YFC0501705)资助。

摘　　要：【目的】探讨矿区不同恢复年限复垦地沙棘人工林土壤真菌群落组成、多样性及其影响因子。【方法】以鄂尔多斯聚鑫龙煤矿复垦区不同恢复年限沙棘人工林为研究对象,撂荒草地为对照,对研究区土样进行采集,通过高通量测序对土样真菌群落测序分析。【结果】研究区共获得真菌8门、30纲、69目、164科和285属,其中子囊菌门和担子菌门为主要菌群,占样地所有菌门的98.2%,不同恢复年限不同土层真菌群落组成存在差异。随着恢复年限的增加,研究区土壤真菌门分类水平上主要类群的个体数及类群数整体呈现增长趋势,整体高于对照样地草地。另外,恢复7年的样地土壤真菌群落丰富度及多样性显著高于其他年限和撂荒草地。冗余分析表明,研究区不同恢复年限沙棘林土壤微生物群落组成主要由土壤pH、土壤有机质含量决定,同时也与土壤氮、磷、钾元素有关。【结论】不同恢复年限沙棘人工林土壤理化性质存在差异,恢复年限的增加提高了真菌的物种丰富度及多样性。[Objective] Soil microorganism is an important component of soil ecosystem and is highly sensitive to environmental changes. The composition, diversity and influencing factors of soil fungal community in Seabuckthorn plantation need to be explored in reclaimed land of mining area at different restoration years. [Methods] The Seabuckthorn plantation with different restoration years in Juxinlong coal mine was taken as the research object and abandoned grassland as the control. The bacterial community in the collected soil samples was analyzed with high flux sequencing. [Results] The results showed that there were differences in the composition of fungal community at different soil layers with different recovery years. There were 8 phyla, 30 classes, 69 orders, 164 families and 285genera of fungi in the study area, and ascomycetes and basidiomycetes were the main flora, accounting for 98.2% of all phyla in the sample plot. With the increase of restoration years, the number of individuals and groups of the main groups at the taxonomic level of soil fungi showed an overall growth trend, which was higher than that of the grassland in the control sample plot. In addition, the richness and diversity of soil fungal communities in the sample plots restored for 7 years were significantly higher than those in other years and abandoned grasslands. Redundancy analysis showed that soil microbial community composition was mainly determined by soil pH and soil organic matter content,and was also related to soil nitrogen, phosphorus and potassium. [Conclusion] The soil physical and chemical properties of Seabuckthorn plantation with different restoration years are different, and the increase of restoration years improves the species richness and diversity of fungi.

关 键 词：高通量测序 不同恢复年限 矿区 沙棘人工林 土壤真菌 

分 类 号：S154[农业科学—土壤学]