人乳头瘤病毒重组细胞模型应用的研究进展  

Research Progress on the Application of A Human Papillomavirus Recombinant Cell Model

作　　者：王馨艺 路一平 孙峥嵘[1] WANG Xinyi;LU Yiping;SUN Zhengrong(Biobank of Shengjing Hospital Affiliated to China Medical University,Shenyang 110000,China)

机构地区：[1]中国医科大学、附属盛京医院、生物样本库,沈阳110000

出　　处：《病毒学报》2023年第1期245-251,共7页Chinese Journal of Virology

基　　金：国家自然科学基金(项目号:82000076),题目:TREM2调节脂肪酸氧化促进肺泡巨噬细胞极化在特发性肺纤维化中的作用机制研究。

摘　　要：人乳头瘤病毒(Human papillomavirus,HPV)为双链环状小DNA病毒,它不仅能引起黏膜上皮的良性增生,也可以导致癌症如宫颈癌、阴茎癌、口咽癌的发生。HPV重组细胞模型的建立及其分化培养,可以应用于HPV早期感染、HPV感染对宿主细胞生命周期影响的研究,也可进一步诱导,用于深入研究DNA损伤修复途径对病毒复制的影响、以及HPV整合机制。本文将主要围绕HPV重组细胞模型的建立、分化培养的方法以及重组细胞模型的应用进行综述。Human papillomavirus(HPV) is a small double-stranded circular DNA virus, that not only causes benign hyperplasia of mucosal epithelium, but also causes cancer cervical, penile, and oropharyngeal cancers.Establishment of an HPV recombinant cell model and analysis of its differentiation in culture can be applied to the study of early HPV infection and the impact of HPV infection on the life cycle of host cells. It may be further introduced to study the effect of DNA damage repair pathways during virus replication, as well as HPV integration mechanisms. This article focused on the establishment of an HPV recombinant cell model, the methods for differentiation and cell culture, and the application of the recombinant cell model.

关 键 词：人乳头瘤病毒 HPV重组细胞模型 分化培养 

分 类 号：R373.9[医药卫生—病原生物学]