乙型肝炎病毒表面抗原和抗体双阳性患者外周血T淋巴细胞亚群分布  

Detection of peripheral blood T lymphocyte subpopulations among patients positive for both hepatitis B virus surface antigen and surface antibody

作　　者：陈丹[1] 王秋波[1] 沈丹丹 CHEN Dan;WANG Qiu-bo;SHEN Dan-dan(Wuxi 9th Affiliated Hospital of Soochow University(Wuxi 9th People’s Hospital),Wuxi,Jiangsu 214121,China;Shanghai Tenth People’s Hospital,Shanghai 200072,China)

机构地区：[1]苏州大学附属无锡九院(无锡市第九人民医院),江苏无锡214121 [2]上海市第十人民医院,上海200072

出　　处：《中国热带医学》2022年第12期1130-1134,共5页China Tropical Medicine

基　　金：国家重点研发计划“国家质量基础的共性技术研究与应用”重点专项子课题(No.2019YFF0216502)。

摘　　要：目的观察乙型肝炎病毒表面抗原(HBsAg)和表面抗体(HBsAb)双阳性患者外周血T淋巴细胞亚群分布。方法以2014—2020年上海市第十人民医院和无锡市第九人民医院收治36例HBsAg和HBsAb双阳性患者为实验组,以同期无锡市第九人民医院40例HBsAg阳性、HBsAb阴性患者作为对照组。采用流式细胞术检测并比较实验组和对照组患者外周血CD3^(+)、CD4^(+)、CD8^(+)T细胞比例,采用Pearson相关分析评价患者外周血T细胞比例及血清谷丙转氨酶(ALT)、谷草转氨酶(AST)水平与血清HBsAb水平间的相关性。结果实验组和对照组患者中位年龄、性别构成、ALT及AST水平、HBV DNA载量>103copies/mL患者比例、HBV E抗原(HBeAg)阳性率、HBV E抗体(HBeAb)阳性率、HBV核心抗体(HBcAb)阳性率差异均无统计学意义(P>0.05)。实验组和对照组患者外周血CD3^(+)[(71.83±1.50)%vs(72.75±1.47)%;t=0.66,P>0.05]、CD4^(+)[(36.81±1.53)%vs(39.88±1.57)%;t=1.43,P>0.05]、CD8^(+)[(33.17±2.04)%vs(32.40±1.75)%;t=0.77,P>0.05]T细胞比例差异均无统计学意义。Pearson相关分析显示,HBsAg、HBsAb双阳性患者血清HB-sAb含量与外周血CD3^(+)(r=0.026,P=0.65)、CD4^(+)(r=-0.08,P=0.16)、CD8^(+)(r=0.09,P=0.24)、CD4^(+)/CD8^(+)T细胞比值(r=-0.005,P=0.35)及ALT(r=0.04,P=0.56)、AST水平(r=0.002,P=0.69)T细胞比例均无显著相关性。结论HBsAg、HBsAb双阳性患者与HBsAg阳性、HBsAb阴性患者外周血CD3^(+)、CD4^(+)、CD8^(+)T细胞亚群比例均无显著差异。Objective To investigate the frequency of peripheral blood T cells among patients positive for both hepatitis B virus surface antigen(HBsAg)and surface antibody(HBsAb).MethodsThirty six patients with co-existence of HBsAg and HBsAb diagnosed were enrolled as the experimental group,who were admitted by Shanghai tenth people′s hospital and Wuxi 9th people′s hospital from 2014 to 2020.while 40 patients tested positive for HBsAg and negative for HBsAb served as controls,who were admitted by Wuxi 9th people′s hospital.Flow cytometry was used to detect and compare the proportions of peripheral blood CD3^(+),CD4^(+)and CD8^(+)T cells between the experimental and control groups.In addition,the associations of serum HBsAb level with peripheral blood T cell proportions,serum alanine aminotransferase(ALT)and aspartate aminotransferase(AST)levels were examined among chronic hepatitis B patients with co-existence of HBsAg and HBsAb using Pearson correlation analysis.ResultsThe median age,gender distribution,mean ALT and AST concentrations,proportion of HBV DNA viral load>10^(3)copies/mL,seroprevalence of HBV E antigen(HBeAg),seroprevalence of HBV E antibody(HBeAb),seroprevalence of HBV core antibody(HBcAb)were comparable between the experimental and control groups,and there were no significant difference in them(P>0.05).There were no significant difference between the experimental and control groups in terms of CD3^(+)T cell proportion[(71.83±1.50)%vs(72.75±1.47)%;t=0.66,P>0.05],CD4^(+)T cell proportion[(36.81±1.53)%vs(39.88±1.57)%;t=1.43,P>0.05]and CD8^(+)T cell proportion[(33.17±2.04)%vs(32.40±1.75)%;t=0.77,P>0.05].Pearson correlation analysis revealed that the serum HBsAb level did not significantly correlate with peripheral blood CD3^(+)(r=0.026,P=0.65),CD4^(+)(r=-0.08,P=0.16)and CD8^(+)T cell proportions(r=0.09,P=0.24),CD4^(+)/CD8^(+)T proportion(r=-0.005,P=0.35),serum ALT(r=0.04,P=0.56)and AST levels(r=0.002,P=0.69)among chronic hepatitis B patients with co-existence of HBsAg and HBsAb.ConclusionsThere are n

关 键 词：乙型肝炎病毒 慢性乙型肝炎 乙型肝炎病毒表面抗原 乙型肝炎病毒表面抗体 外周血T细胞 

分 类 号：R512.62[医药卫生—内科学]