核糖体小亚基V4和V9区引物扩增大亚湾真核浮游生物的比较研究  

Comparison of eukaryotic plankton compositions using 18S rRNA gene V4and V9 primers amplification in Daya Bay

作　　者：郭雨沛 黄良民[1,2] 钟瑜 陈靖夫[1,2] 谭烨辉 陈绵润[3] 邱大俊 GUO Yupei;HUANG Liangmin;ZHONG Yu;CHEN Jingfu;TAN Yehui;CHEN Mianrun;Qiu Dajun(CAS Key Laboratory of Tropical Marine Bio-resources and Ecology,South China Sea Institute of Oceanology,Chinese Academy of Sciences,Guangzhou 510301,China;University of Chinese Academy of Sciences,Beijing 101408,China;South China Sea Institute of Planning and Environmental Research,SOA,Guangzhou 510300,China)

机构地区：[1]中国科学院南海海洋研究所,中科院热带生物资源与生态重点实验室,广州510301 [2]中国科学院大学,北京101408 [3]国家海洋局南海规划与环境研究院,广州510300

出　　处：《生态科学》2023年第1期242-251,共10页Ecological Science

基　　金：国家自然科学基金(42276165,41776154);广州海洋实验室2019年度人才团队引进项目(GML2019ZD0405)。

摘　　要：对大亚湾水体的环境DNA分别进行18S rDNA的V4和V9区的引物扩增,通过高通量测序技术进行测序,并比较分析二者浮游真核生物基因多样性和相对丰度。18S rDNA V4区引物扩增共检测出浮游动物56纲,101目,浮游植物52纲,69目;18S rDNA V9区引物扩增共检测出浮游动物47纲,81目,浮游植物56纲,101目。两对引物对浮游真核生物扩增都具有较高覆盖度,在纲级别上二者的结果相近:颚足纲(Maxillopoda)是浮游动物优势类群;甲藻纲(Dinophyceae)、圆筛藻纲(Coscinodiscophyceae)、小豆藻纲(Mamiellophyceae)是浮游植物优势类群,其中甲藻纲多样性与丰度的结果相近,而18S rDNA V9区引物扩增得到的圆筛藻纲丰度高于18S rDNA V4区引物。分析结果表明,18S rDNAV4区引物扩增的浮游动物多样性比18SrDNAV9区引物高,而18SrDNAV9区引物扩增的浮游植物多样性比18S rDNA V4区引物高。同时,通过高通量测序技术首次确定大亚湾海区大量存在着寄生型甲藻(Syndiniales),小豆藻目(Mamiellales)。We compared the genetic diversity and relative abundance of eukaryotic plankton in Daya Bay using two genetic markers(18S rRNA V4 and V9 regions) to amplify the eDNA and high-throughput sequencing technology. 56 classes, 101 orders of zooplankton and 52 classes, 69 orders of phytoplankton were detected using 18S rDNA V4 markers. And 47 classes, 81 orders of zooplankton and 56 classes, 101 orders of phytoplankton were detected using 18S rDNA V9 markers. Both 18S rDNA V4 and V9regions results performed well to coverage communities and provided similar eukaryotic plankton distribution patterns at class taxonomic level. Maxillopoda is the dominant group of zooplankton, while Dinophyceae, Coscinodiscophyceae and Mamiellophyceae are the dominant groups of phytoplankton. Especially, two genetic markers amplification results present the similar diversity and relative abundance of Dinophyceae at order taxonomic level. The results of 18S rDNA V9 region presented higher abundance of Coscinodiscophyceae than that of V4. The results showed that the diversity of zooplankton amplification by 18S rDNA V4 primers was higher than that of 18S rDNA V9 primers, while that of phytoplankton amplification by 18S rDNA V9primers was higher than that of 18S rDNA V4 primers. In this study, we first report high abundance parasitic Syndiniales and Mamiellales in Daya Bay.

关 键 词：高通量测序 真核浮游生物 基因多样性 浮游植物 浮游动物 大亚湾 

分 类 号：Q178.53[生物学—水生生物学] Q7[生物学—普通生物学]